Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Melinda Hauser"'
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 9, Iss 8, Pp 2549-2560 (2019)
We determined the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 min after recovery in growth medium. Laser-irradiated cells were viable, and th
Externí odkaz:
https://doaj.org/article/9d3fec52e504424eafe95770a0f456cf
Autor:
Seth T Langford, Cody S Wiggins, Roque Santos, Melinda Hauser, Jeffrey M Becker, Arthur E Ruggles
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0180503 (2017)
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated t
Externí odkaz:
https://doaj.org/article/d3cb4d57d2644a1ab9ba92711935d51c
Publikováno v:
Bio-Protocol, Vol 6, Iss 22 (2016)
We describe an assay for determination of toxicity in S. cerevisiae involving spotting of a toxic peptide on a lawn of yeast cells. This assay may be generalized to determine toxicity of a variety of compounds by substituting a putative toxic compoun
Externí odkaz:
https://doaj.org/article/8da26034ef3f41fd802b962f84fe8070
Publikováno v:
Bio-Protocol, Vol 6, Iss 22 (2016)
We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via specific car
Externí odkaz:
https://doaj.org/article/e1cf6d860ad24a1fb881c0f2fa2a6cf0
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 9, Iss 8, Pp 2549-2560 (2019)
We determined the effect on gene transcription of laser-mediated, long-wavelength UV-irradiation of Saccharomyces cerevisiae by RNAseq analysis at times T15, T30, and T60 min after recovery in growth medium. Laser-irradiated cells were viable, and th
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1859:698-711
Dimerization of G protein-coupled receptors (GPCR) may play an important role in maturation, internalization, signaling and/or pharmacology of these receptors. However, the location where dimerization occurs is still under debate. In our study, varia
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1858(4):715-724
G protein-coupled receptors (GPCRs) are found in all eukaryotic cells examined to date where they function as membrane-bound proteins that bind a multitude of extracellular ligands to initiate intracellular signal transduction systems controlling cel
Autor:
Roque Santos, Melinda Hauser, Jeffrey M. Becker, Arthur E. Ruggles, Seth Langford, Cody Wiggins
Publikováno v:
PLoS ONE
PLoS ONE, Vol 12, Iss 7, p e0180503 (2017)
PLoS ONE, Vol 12, Iss 7, p e0180503 (2017)
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated t
Publikováno v:
Bio-protocol. 6(22)
We describe an assay for determination of toxicity in S. cerevisiae involving spotting of a toxic peptide on a lawn of yeast cells. This assay may be generalized to determine toxicity of a variety of compounds by substituting a putative toxic compoun
Autor:
Melinda Hauser, Steven T. King, Chen Qian, Jeffrey M. Becker, Fred Naider, Robert L. Hettich, Sarah Kauffman
Publikováno v:
Journal of molecular recognition : JMR. 31(2)
We are developing a rapid, time-resolved method using laser-activated cross-linking to capture protein-peptide interactions as a means to interrogate the interaction of serum proteins as delivery systems for peptides and other molecules. A model syst