Rolling circle amplification of synthetic DNA accelerates biocatalytic determination of enzyme activity relative to conventional methods.

Autor: Hadi T; GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, Pennsylvania, 19426, USA., Nozzi N; GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, Pennsylvania, 19426, USA., Melby JO; GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, Pennsylvania, 19426, USA., Gao W; GE Global Research, One Research Circle, Niskayuna, NY, 12309, USA., Fuerst DE; GlaxoSmithKline, 1250 South Collegeville Road, Collegeville, Pennsylvania, 19426, USA., Kvam E; GE Global Research, One Research Circle, Niskayuna, NY, 12309, USA. kvame@ge.com.

Publikováno v: Scientific reports [Sci Rep] 2020 Jun 24; Vol. 10 (1), pp. 10279. Date of Electronic Publication: 2020 Jun 24.

Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso Peptide from an understudied halophilic actinomycete.

Autor: Metelev M; Institute for Nanobiotechnologies, Saint Petersburg State Polytechnical University, Saint Petersburg 195251, Russia; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Tietz JI; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Melby JO; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Blair PM; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Zhu L; NMR Laboratory, School of Chemical Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Livnat I; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Severinov K; Institute for Nanobiotechnologies, Saint Petersburg State Polytechnical University, Saint Petersburg 195251, Russia; Department of Molecular Biology and Biochemistry, Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA., Mitchell DA; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: douglasm@illinois.edu.

Publikováno v: Chemistry & biology [Chem Biol] 2015 Feb 19; Vol. 22 (2), pp. 241-50. Date of Electronic Publication: 2015 Jan 15.

Structural investigation of ribosomally synthesized natural products by hypothetical structure enumeration and evaluation using tandem MS.

Autor: Zhang Q; Howard Hughes Medical Institute,Departments of Chemistry., Ortega M; Howard Hughes Medical Institute,Biochemistry, and., Shi Y; Howard Hughes Medical Institute,Departments of Chemistry., Wang H; Howard Hughes Medical Institute,Departments of Chemistry., Melby JO; Departments of Chemistry,Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Tang W; Howard Hughes Medical Institute,Departments of Chemistry., Mitchell DA; Departments of Chemistry,Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801Microbiology, and., van der Donk WA; Howard Hughes Medical Institute,Departments of Chemistry,Biochemistry, andInstitute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 vddonk@illinois.edu.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2014 Aug 19; Vol. 111 (33), pp. 12031-6. Date of Electronic Publication: 2014 Aug 04.

Engineering unnatural variants of plantazolicin through codon reprogramming.

Autor: Deane CD; Department of Chemistry, ‡Institute for Genomic Biology, and §Department of Microbiology, ∥School of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States., Melby JO, Molohon KJ, Susarrey AR, Mitchell DA

Publikováno v: ACS chemical biology [ACS Chem Biol] 2013 Sep 20; Vol. 8 (9), pp. 1998-2008. Date of Electronic Publication: 2013 Jul 03.

YcaO domains use ATP to activate amide backbones during peptide cyclodehydrations.

Autor: Dunbar KL; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Melby JO, Mitchell DA

Publikováno v: Nature chemical biology [Nat Chem Biol] 2012 Apr 22; Vol. 8 (6), pp. 569-75. Date of Electronic Publication: 2012 Apr 22.