KnowVolution of a Fungal Laccase toward Alkaline pH

Autor: Ulrich Schwaneberg, Jasmin Eidner, Gaurao V. Dhoke, Ronny Martinez, Catalina Novoa, Patrick Lorenz, Diana M. Mate, Ruth Schwerdtfeger, Mehdi D. Davari, Thomas Haarmann, Felix Jakob, Martina Schreiter

Publikováno v: ChemBioChem. 20:1458-1466

To date, commercial laccase preparations are used in the food, textile, and paper and pulp industries (mild pH). Laccases are attractive in the synthesis of dye molecules or oxidative lignin treatment, which take place at high pH (≥8.0). So far, on

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f921a3fa760a1ff9bb7782e12ca3934
https://doi.org/10.1002/cbic.201800807

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site

Autor: Laura-Leena Kiiskinen, Markku Saloheimo, Anu Koivula, Kristiina Kruus, Nina Hakulinen, Arja Paananen, Juha Rouvinen

Publikováno v: Hakulinen, N, Kiiskinen, L-L, Kruus, K, Saloheimo, M, Paananen, A, Koivula, A & Rouvinen, J 2002, ' Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site ', Nature Structural Biology, vol. 9, no. 8, pp. 601-605 . https://doi.org/10.1038/nsb823

We have crystallized the ascomycete laccase from Melanocarpus albomyces with all four coppers present and determined the crystal structure at 2.4 Å resolution. The enzyme is heavily glycosylated and consists of three cupredoxin-like domains, similar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bfc2577fa351032b237065fa28f1fe2
https://doi.org/10.1038/nsb823

Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces

Autor: Jari Vehmaanperä, Matti Siika-aho, Kati Réczey, Marika Alapuranen, Nóra Szijártó, Liisa Viikari, Maija Tenkanen

Publikováno v: Szijártó, N, Siika-aho, M, Tenkanen, M, Alapuranen, M, Vehmaanperä, J, Réczey, K & Viikari, L 2008, ' Hydrolysis of amorphous and crystalline cellulose by heterologously produced cellulases of Melanocarpus albomyces ', Journal of Biotechnology, vol. 136, no. 3-4, pp. 140-147 . https://doi.org/10.1016/j.jbiotec.2008.05.010

Three thermostable neutral cellulases from Melanocarpus albomyces, a 20-kDa endoglucanase (Cel45A), a 50-kDa endoglucanase (Cel7A), and a 50-kDa cellobiohydrolase (Cel7B) heterologously produced in a recombinant Trichoderma reesei were purified and s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca109b6d268d1785b1525da0834023e3
https://doi.org/10.1016/j.jbiotec.2008.05.010

Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability

Autor: Jari Vehmaanperä, Harry Boer, Markus Linder, Juha Rouvinen, Anu Koivula, Sanni Voutilainen, Terhi Puranen

Publikováno v: Voutilainen, S, Boer, H, Linder, M, Puranen, T, Rouvinen, J, Vehmaanperä, J & Koivula, A 2007, ' Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability ', Enzyme and Microbial Technology, vol. 41, no. 3, pp. 234-243 . https://doi.org/10.1016/j.enzmictec.2007.01.015

Fungal cellobiohydrolases from the glycosyl hydrolase family 7 are key enzymes in crystalline cellulose hydrolysis. Difficulties in heterologous expression in a bacterial or yeast host have hampered engineering of these cellulases for industrial appl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55d57b122b0e543f9bd044abe3526bdf
https://cris.vtt.fi/en/publications/56a9b1ed-0481-4e76-aa96-5e7e43e0d0ad