A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.

Autor: Karamjit Singh Dolt, Melanie L Lawrence, Eve Miller-Hodges, Joan Slight, Anna Thornburn, Paul S Devenney, Peter Hohenstein

Publikováno v: PLoS ONE, Vol 8, Iss 4, p e62054 (2013)

There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC tr

Externí odkaz: https://doaj.org/article/b314354c8ae344129f90f860e3a09364

Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture

Autor: Mona Elhendawi, Melanie L. Lawrence, Jamie A. Davies

Publikováno v: Methods in Molecular Biology ISBN: 9781493990207

Some aspects of renal physiology, in particular transport across tubular epithelia, are highly relevant to pharmacokinetics and to drug toxicity. The use of animals to model human renal physiology is limited, but human-derived renal organoids offer a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8188f29c6d0b4cd7f88e579ad418b0aa
https://doi.org/10.1007/978-1-4939-9021-4_11

The Sequence-specific Peptide-binding Activity of the Protein Sulfide Isomerase AGR2 Directs Its Stable Binding to the Oncogenic Receptor EpCAM

Autor: Melanie L. Lawrence, Borek Vojtesek, Ted R. Hupp, J. Robert O’Neill, Euan Murray, M. Aiman Mohtar, Lenka Hernychová

Publikováno v: Molecular & Cellular Proteomics : MCP
Mohtar, M A, Hernychova, L, O'Neill, J, Lawrence, L, Murray, E, Vojtesek, B & Hupp, T R 2018, ' The sequence-specific peptide-binding activity of the protein sulfide isomerase AGR2 directs its stable binding to the oncogenic receptor EpCAM ', Molecular and Cellular Proteomics . https://doi.org/10.1074/mcp.RA118.000573

AGR2 is an oncogenic endoplasmic reticulum (ER)-resident protein disulfide isomerase. AGR2 protein has a relatively unique property for a chaperone in that it can bind sequence-specifically to a specific peptide motif (TTIYY). A synthetic TTIYY-conta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fce97c3dc4734626660eae867bebe4e0
https://pubmed.ncbi.nlm.nih.gov/29339412

From organoids to mini-organs

Autor: Melanie L. Lawrence, Jamie A. Davies, Christopher G. Mills

Development of the permanent mammalian kidney, the metanephros, relies on complex reciprocal interactions between two nephrogenic cell populations; the branching ureteric bud and the metanephrogenic mesenchyme. Exploiting these interactions in vitro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7c9551363d96b4969ea2c93815b9cc16
https://doi.org/10.1016/b978-0-12-812636-3.00009-2

Transport of organic anions and cations in murine embryonic kidney development and in serially-reaggregated engineered kidneys

Autor: C-Hong Chang, Jamie A. Davies, Melanie L. Lawrence

Publikováno v: Lawrence, M L, Chang, C-H & Davies, J A 2015, ' Transport of organic anions and cations in murine embryonic kidney development and in serially-reaggregated engineered kidneys ', Scientific Reports, vol. 5, pp. 9092 . https://doi.org/10.1038/srep09092
Scientific Reports

Recent advances in renal tissue engineering have shown that dissociated, early renogenic tissue from the developing embryo can self-assemble into morphologically accurate kidney-like organs arranged around a central collecting duct tree. In order for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bccf0ea4aeed86aec0f69a58fcfebc6