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Autor:
Andreas Burkovski, Reinhard Krämer, Gregor Wersch, Melanie Finkemeier, Walter Pfefferle, Thomas Hermann
Publikováno v:
Electrophoresis. 21:654-659
An improved protocol for the two-dimensional analysis of proteins of the Corynebacterium glutamicum cytoplasmic membrane fraction is described. By use of increased 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) concentrations (2-4%