The GET insertase exhibits conformational plasticity and induces membrane thinning

Autor: Melanie A. McDowell, Michael Heimes, Giray Enkavi, Ákos Farkas, Daniel Saar, Klemens Wild, Blanche Schwappach, Ilpo Vattulainen, Irmgard Sinning

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)

Abstract The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone captures the TA protein substrate and delive

Externí odkaz: https://doaj.org/article/b6447c79d60b44f2a61ff200ac48153d

Functional analysis of the interdependence between DNA uptake sequence and its cognate ComP receptor during natural transformation in Neisseria species.

Autor: Jamie-Lee Berry, Ana Cehovin, Melanie A McDowell, Susan M Lea, Vladimir Pelicic

Publikováno v: PLoS Genetics, Vol 9, Iss 12, p e1004014 (2013)

Natural transformation is the widespread biological process by which "competent" bacteria take up free DNA, incorporate it into their genomes, and become genetically altered or "transformed". To curb often deleterious transformation by foreign DNA, s

Externí odkaz: https://doaj.org/article/042852504f18431baa5dcd62913c8fa3

Structural Basis of Tail-Anchored Membrane Protein Biogenesis by the GET Insertase Complex

Autor: Volker Schmid, Melanie A. McDowell, Michael Heimes, Ákos Farkas, Francesco Fiorentino, Jani Reddy Bolla, Shahid Mehmood, Klemens Wild, Dirk Flemming, Stefan Pfeffer, Blanche Schwappach, Javier Coy-Vergara, Roger Heinze, Carol V. Robinson, Irmgard Sinning, Di Wu

Summary Membrane protein biogenesis faces the challenge of chaperoning hydrophobic transmembrane helices for faithful membrane insertion. The guided entry of tail-anchored proteins (GET) pathway targets and inserts tail-anchored (TA) proteins into th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44520d0096748fb3fe31d7a79cd540fb
http://hdl.handle.net/11573/1561739

Specific DNA recognition mediated by a type IV pilin

Autor: Stephen Matthews, Ana Cehovin, Daniel R. Brown, Susan M. Lea, Geoffrey S. Baldwin, Rossella Noschese, Vladimir Pelicic, Melanie A. Mcdowell, Peter Simpson, Mitchell A. Pallett, Jacob P. Brady

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2013, 110 (8), pp.3065-3070. ⟨10.1073/pnas.1218832110⟩

Natural transformation is a dominant force in bacterial evolution by promoting horizontal gene transfer. This process may have devastating consequences, such as the spread of antibiotic resistance or the emergence of highly virulent clones. However,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b69a68a80f332951ed52d19ce120d1d
https://doi.org/10.1073/pnas.1218832110

Building a secreting nanomachine: a structural overview of the T3SS

Autor: Patrizia, Abrusci, Melanie A, McDowell, Susan M, Lea, Steven, Johnson

Publikováno v: Current Opinion in Structural Biology

Graphical abstract
Highlights • Flagellar and non-flagellar T3SS are built assembling homologous protein machineries. • Unified nomenclature for non-flagellar T3SS. • New model of the T3SS needle is consistent with the flagellar filament,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f010f0ee7da9d979f32214d4c9a39c9f
https://pubmed.ncbi.nlm.nih.gov/24704748