Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Meiyappan Lakshmanan"'
Autor:
Meiyappan Lakshmanan, Sean Chia, Kuin Tian Pang, Lyn Chiin Sim, Gavin Teo, Shi Ya Mak, Shuwen Chen, Hsueh Lee Lim, Alison P. Lee, Farouq Bin Mahfut, Say Kong Ng, Yuansheng Yang, Annie Soh, Andy Hee-Meng Tan, Andre Choo, Ying Swan Ho, Terry Nguyen-Khuong, Ian Walsh
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 23, Iss , Pp 2497-2506 (2024)
N-glycosylation can have a profound effect on the quality of mAb therapeutics. In biomanufacturing, one of the ways to influence N-glycosylation patterns is by altering the media used to grow mAb cell expression systems. Here, we explore the potentia
Externí odkaz:
https://doaj.org/article/8c61532e278a4848aa34e06e0575d137
Autor:
Valériane Malika Keita, Yi Qing Lee, Meiyappan Lakshmanan, Dave Siak-Wei Ow, Paul Staniland, Jessica Staniland, Ian Savill, Kang Lan Tee, Tuck Seng Wong, Dong-Yup Lee
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-17 (2024)
Abstract Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the f
Externí odkaz:
https://doaj.org/article/405144ec2f70414e94c01bea0a8ed6d3
Mitigating biomass composition uncertainties in flux balance analysis using ensemble representations
Autor:
Yoon-Mi Choi, Dong-Hyuk Choi, Yi Qing Lee, Lokanand Koduru, Nathan E. Lewis, Meiyappan Lakshmanan, Dong-Yup Lee
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 3736-3745 (2023)
The biomass equation is a critical component in genome-scale metabolic models (GEMs): it is used as the de facto objective function in flux balance analysis (FBA). This equation accounts for the quantities of all known biomass precursors that are req
Externí odkaz:
https://doaj.org/article/0a884a6507744c7babcf42477f68670a
Autor:
Ian Walsh, Matthew Myint, Terry Nguyen-Khuong, Ying Swan Ho, Say Kong Ng, Meiyappan Lakshmanan
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
Ensuring consistent high yields and product quality are key challenges in biomanufacturing. Even minor deviations in critical process parameters (CPPs) such as media and feed compositions can significantly affect product critical quality attributes (
Externí odkaz:
https://doaj.org/article/92ace70090b442b389f8aa84b7020cd6
Autor:
Lokanand Koduru, Meiyappan Lakshmanan, Shawn Hoon, Dong-Yup Lee, Yuan Kun Lee, Dave Siak-Wei Ow
Publikováno v:
Frontiers in Microbiology, Vol 13 (2022)
The incidence and prevalence of inflammatory disorders have increased globally, and is projected to double in the next decade. Gut microbiome-based therapeutics have shown promise in ameliorating chronic inflammation. However, they are largely experi
Externí odkaz:
https://doaj.org/article/b8c41423612f4c6c9344cde186afbac3
Autor:
Nana Y. D. Ankrah, David B. Bernstein, Matthew Biggs, Maureen Carey, Melinda Engevik, Beatriz García-Jiménez, Meiyappan Lakshmanan, Alan R. Pacheco, Snorre Sulheim, Gregory L. Medlock
Publikováno v:
mSystems, Vol 6, Iss 6 (2021)
ABSTRACT Construction and analysis of genome-scale metabolic models (GEMs) is a well-established systems biology approach that can be used to predict metabolic and growth phenotypes. The ability of GEMs to produce mechanistic insight into microbial e
Externí odkaz:
https://doaj.org/article/a192a55b16ec410b8f2545d80efb061b
Publikováno v:
Microbial Cell Factories, Vol 17, Iss 1, Pp 1-12 (2018)
Abstract Background Cellular metabolism is tightly regulated by hard-wired multiple layers of biological processes to achieve robust and homeostatic states given the limited resources. As a result, even the most intuitive enzyme-centric metabolic eng
Externí odkaz:
https://doaj.org/article/238318946ea24da7bd64cb630c054822
Autor:
Murukarthick Jayakodi, Beom-Soon Choi, Sang-Choon Lee, Nam-Hoon Kim, Jee Young Park, Woojong Jang, Meiyappan Lakshmanan, Shobhana V. G. Mohan, Dong-Yup Lee, Tae-Jin Yang
Publikováno v:
BMC Plant Biology, Vol 18, Iss 1, Pp 1-7 (2018)
Abstract Background The ginseng (Panax ginseng C.A. Meyer) is a perennial herbaceous plant that has been used in traditional oriental medicine for thousands of years. Ginsenosides, which have significant pharmacological effects on human health, are t
Externí odkaz:
https://doaj.org/article/b38e4088d25145da8fd2f7b606d947ec
Publikováno v:
Frontiers in Plant Science, Vol 7 (2016)
Crop productivity is severely limited by various biotic and abiotic stresses. Thus, it is highly needed to understand the underlying mechanisms of environmental stress response and tolerance in plants, which could be addressed by systems biology appr
Externí odkaz:
https://doaj.org/article/3e437bed02614c22bc95e7e2570840bf
Publikováno v:
Biotechnology and Bioengineering. 119:1740-1754
Chinese hamster ovary (CHO) cells are widely used for producing recombinant proteins. To enhance their productivity and product quality, media reformulation has been a key strategy, albeit with several technical challenges, due to the myriad of compl