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of 12
pro vyhledávání: '"Meir Fischer"'
Autor:
Yoffi Segall, Hendrik P. Benschop, Avigdor Shafferman, Arie Ordentlich, Leo P. A. De Jong, Baruch Velan, Meir Fischer, Ruth Barak, Dov Barak
Publikováno v:
FEBS Letters, 3, 407, 347-352
While non-reactivability of cholinesterases from their phosphyl conjugates (aging) is attributed to an unimolecular process involving loss of alkyl group from the phosphyl moiety, no conclusive evidence is available that this is the only reaction pat
Expression and reconstitution of biologically active human acetylcholinesterase fromEscherichia coli
Publikováno v:
Cellular and Molecular Neurobiology. 13:25-38
1. Authentic human acetylcholinesterase (AChE) was expressed inEscherichia coli under regulation of the constitutivedeo promoter or the thermoinducibleλPL promoter. 2. To facilitate expression in the prokaryotic system, recombinant human AChE (rhACh
Autor:
Lola Weiss, Shimon Slavin, Jacob R. Hartman, Zelig Tochner, Abraham Nimrod, Meir Fischer, Yaffa Beck, Marian Gorecki
Publikováno v:
Free Radical Research Communications. 12:401-410
In many pathological situations, tissue damage is caused by cellular generation of superoxide free radicals (O2-). These active species are generated during post-ischemic reperfusion of organs, in hyperoxic tissue, during acute and chronic inflammati
ESMS as a Unique Tool for the Molecular Monitoring of Reactions between HuAChE and Various OP-Agents
Autor:
Baruch Velan, Arie Ordentlich, Avigdor Shafferman, Meir Fischer, H. P. Benschop, Dov Barak, Ruth Barak, Leo P. A. De Jong, Yoffi Segall
Publikováno v:
Structure and Function of Cholinesterases and Related Proteins ISBN: 9781489915429
The molecular masses of the bacterially expressed recombinant HuAChE and its conjugates with series of alkyl methylphosphonofluoridates and with diisopropyl fluorophosphate (DFP), were measured by electrospray - ionization mass spectrometry (ESMS). T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2c37154f0691e2a6636a4c13ba7754b9
https://doi.org/10.1007/978-1-4899-1540-5_74
https://doi.org/10.1007/978-1-4899-1540-5_74
Autor:
Meir Fischer, Ilana Liefer, Marian Gorecki, Ruth Lifshitz, Elisha Zeelon, Amos Panet, Tova Katz, Hana Ben-Artzi
Publikováno v:
Protein expression and purification. 3(4)
To study the subunit structure and the active site of human immunodeficiency virus reverse transcriptase (RT), the enzyme was expressed in E. coli and purified to homogeneity in large quantities. The recombinant enzyme consists of two major polypepti
Publikováno v:
Soil Biology and Biochemistry. 18:69-75
The adsorption of Azospirillum brasilense strain Cd to corn roots was measured using 32 P-labeled Azospirillum cells. Binding kinetics showed that adherence of bacteria to roots increased during the first 90 min and attained a maximum level within 4.
Publikováno v:
Soil Biology and Biochemistry. 20:45-49
Root surface area of maize seedlings was the most reliable criterion in evaluation and measurement of the growth responses of maize inoculated with Azospirillum . The optimal concentration of Azospirillum inoculum for increasing root surface areas wa
Publikováno v:
Soil Biology and Biochemistry. 21:147-153
Inoculation of maize seedlings with 107 colony forming units (cfu) of Azospirillum plant−1 significantly increased the root surface area 2 weeks after sowing as compared to non-inoculated plants. Indole-3-acetic acid (IAA) was identified by gas liq
Autor:
Meir Fischer, Drew Schwartz
Publikováno v:
Moleculargeneral genetics : MGG. 127(1)
Maize alcohol dehydrogenase (ADH1) has been dissociated and reassociated in vitro. The results support previous genetic data on the dimeric structure of the enzyme. Our studies reveal interesting differences between the dissociation and reassociation
Autor:
Meir Fischer, Drew Schwartz
Publikováno v:
Biochimica et biophysica acta. 364(2)
In vitro, alcohol:NAD + oxidoreductase (EC 1.1.1.1) monomers of maize associate into transient inactive oligomers. The immature aggregate is formed when the subunits reassociate at low temperature in the presence of zinc ions. A rapid transition occu