Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Mehmet, Berkmen"'
Publikováno v:
Heliyon, Vol 10, Iss 5, Pp e26996- (2024)
Background: Heterologous expression of active, native-folded protein in Escherichia coli is critical in both academic research and biotechnology settings. When expressing non-native recombinant proteins in E. coli, obtaining soluble and active protei
Externí odkaz:
https://doaj.org/article/8b096a31ab7540fdb7c33f3e643a101a
Autor:
Michael-Paul Robinson, Jinjoo Jung, Natalia Lopez-Barbosa, Matthew Chang, Mingji Li, Thapakorn Jaroentomeechai, Emily C. Cox, Xiaolu Zheng, Mehmet Berkmen, Matthew P. DeLisa
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on the transport of a bif
Externí odkaz:
https://doaj.org/article/de5dd8828395498fa9ebf9a6b2a097c0
Autor:
Paul Riggs, George Blundell-Hunter, Joanna Hagelberger, Guoping Ren, Laurence Ettwiller, Mehmet Berkmen
Publikováno v:
Frontiers in Molecular Biosciences, Vol 8 (2021)
Transposable elements (TE) are mobile genetic elements, present in all domains of life. They commonly encode a single transposase enzyme, that performs the excision and reintegration reactions, and these enzymes have been used in mutagenesis and crea
Externí odkaz:
https://doaj.org/article/de574cf1272642078eaddcdfe0505d77
Autor:
Witsanu Srila, Thae Thae Min, Thitima Sumphanapai, Kuntalee Rangnoi, Mehmet Berkmen, Montarop Yamabhai
Publikováno v:
Applied Microbiology and Biotechnology. 107:1959-1970
Abstract Efficient selection and production of antibody fragments in microbial systems remain to be a challenging process. To optimize microbial production of single-chain variable fragments (scFvs), we have chosen five model targets, 1) a hapten, Ze
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07a8f7b2b11178745bc38c16b2a02723
https://doi.org/10.1007/s00253-023-12395-6
https://doi.org/10.1007/s00253-023-12395-6
Autor:
Wilhad Hans Reuter, Thorsten Masuch, Na Ke, Marine Lenon, Meytal Radzinski, Vu Van Loi, Guoping Ren, Paul Riggs, Haike Antelmann, Dana Reichmann, Lars I. Leichert, Mehmet Berkmen
Publikováno v:
Redox Biology, Vol 26, Iss , Pp - (2019)
Understanding the in vivo redox biology of cells is a complex albeit important biological problem. Studying redox processes within living cells without physical disruption or chemical modifications is essential in determining the native redox states
Externí odkaz:
https://doaj.org/article/b5d4ecd420124a238d0ac4ddb8642c1e
Publikováno v:
FEMS Microbiology Letters. 370
Living art made with bacteria is gaining global attention, spreading from laboratories into the public domain: from school STEAM (Science, Technology, Engineering, the Arts, and Mathematics) events to art galleries, museums, community labs, and ultim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::982cf58aaf05f9542532455a55b24234
https://doi.org/10.1093/femsle/fnad025
https://doi.org/10.1093/femsle/fnad025
Autor:
Mehmet Berkmen, Marine Lénon, Guoping Ren, Megan E. Meuser, Patrick J. Loll, Paul Riggs, Na Ke
Publikováno v:
Protein Sci
Maltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross‐reactivity to other proteins in an Escherichia coli lysate. This high level of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a30a3c6c4709caa6b91c02f0e0ec2c59
https://doi.org/10.1002/pro.4088
https://doi.org/10.1002/pro.4088
Autor:
Witsanu Srila, Thae Thae Min, Thitima Sumphanapai, Kuntalee Rangnoi, Mehmet Berkmen, Montarop Yamabhai
Publikováno v:
Applied Microbiology and Biotechnology. 107:3129-3129
The article “Production and applications of fluorobody from redox‑engineered Escherichia coli” was originally published Online First without open access. After publication in volume 107, issue 5-6, page 1959–1970, the author decided to opt fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::318a9287b33556e104569173c09d14d1
https://doi.org/10.1007/s00253-023-12494-4
https://doi.org/10.1007/s00253-023-12494-4
Autor:
Bruno Manta, Hassan Sakhtah, Guoping Ren, Bryce Causey, Cecily Szady, Mehmet Berkmen, Marine Lénon, Na Ke
Publikováno v:
Applied Microbiology and Biotechnology
Abstract Microbial production of antibodies offers the promise of cheap, fast, and efficient production of antibodies at an industrial scale. Limiting this capacity in prokaryotes is the absence of the post-translational machinery, present in dedicat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60cf8c2a9d7e6cf492b62bd9e8a516c0
http://europepmc.org/articles/PMC7595990
http://europepmc.org/articles/PMC7595990
