Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Mehmedalija Jahic"'
Publikováno v:
Biotechnology Progress. 22:1465-1473
Developments in process techniques for production and recovery of heterologous proteins with Pichia pastoris are presented. Limitations for the standard techniques are described, and alternative techniques that solve the limitations problems are revi
Autor:
Mehmedalija Jahic, Andres Veide, Sven-Olof Enfors, Mariena Ketudat-Cairns, Theppanya Charoenrat
Publikováno v:
Biochemical Engineering Journal. 30:205-211
Two strategies to increase the productivity of secreted Thai Rosewood beta-glucosidase in Pichia pastoris processes were evaluated. Both methods were based on increasing the oxygen transfer rate (O ...
Publikováno v:
Biotechnology Progress. 22:1465-1473
Autor:
Tuula T. Teeri, Mehmedalija Jahic, Sven-Olof Enfors, Hongbin Henriksson, Monika Bollok, Åsa M. Kallas
Publikováno v:
Applied Biochemistry and Biotechnology. 126:61-77
The gene XET16A encoding the enzyme xyloglucan endotransglycosylase (XET) from hybrid aspen (Populus tremula x tremuloides Mich) was transformed into Pichia pastoris GS115 and the enzyme was secreted to the medium. The influence of process conditions
Publikováno v:
Bioprocess and Biosystems Engineering. 24:385-393
A fusion protein composed of a cellulose binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B (CBD-lipase) was produced by Pichia pastoris methanol utilization plus phenotype in high cell-density cultures. The ge
Autor:
L. Boone, S. Hjort, Britta Jürgen, A. Manelius, Bo Xu, Laszlo Fuchs, Naruemol Noisommit-Rizzi, P. C. Friberg, Peter Neubauer, Peter Vrábel, G. Hamer, Harald Skogman, Frans W. J. M. M. Hoeks, Bjørn H. Hjertager, Alvin W. Nienow, Caroline M. McFarlane, Christian Trägårdh, A. Rozkov, H. Y. Lin, Matthias Reuss, Michael Hecker, G. Blomsten, R. G. J. M. van der Lans, Karel Ch. A. M. Luyben, Sven-Olof Enfors, Tamas Kovacs, Mehmedalija Jahic, Johan Revstedt, D. O'Beirne, Elke Krüger, Thomas Schweder, Christopher J. Hewitt
Publikováno v:
Journal of Biotechnology. 85:175-185
Escherichia coli fed-batch cultivations at 22 m3 scale were compared to corresponding laboratory scale processes and cultivations using a scale-down reactor furnished with a high-glucose concentration zone to mimic the conditions in a feed zone of th
Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
Autor:
José Luis Montesinos, Mehmedalija Jahic, Francisco Valero, Anna Surribas, Sven-Olof Enfors, Rainer Stahn
Publikováno v:
Journal of biotechnology. 130(3)
Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at earl
Publikováno v:
Microbial Cell Factories, Vol 5, Iss Suppl 1, p P24 (2006)
For improved interfacing of the Pichia pastoris fed-batch cultivation process with expanded bed adsorption (EBA) technique, a modified cultivation technique was developed. The modification included the reduction of the medium salt concentration, whic
Autor:
Andres Veide, Sven-Olof Enfors, Mehmedalija Jahic, Mariena Ketudat-Cairns, Theppanya Charoenrat
Publikováno v:
Journal of biotechnology. 122(1)
Methanol limited fed-batch cultivation was applied for production of a plant derived beta-glucosidase by Pichia pastoris. The beta-glucosidase was recovered by expanded bed adsorption chromatography applied to the whole culture broth. The new Streaml
Autor:
Helle Stendahl-Andersen, Sven-Olof Enfors, Theppanya Charoenrat, Mehmedalija Jahic, Mariena Ketudat-Cairns
Publikováno v:
Bioprocess and biosystems engineering. 27(6)
An oxygen-limited fed-batch technique (OLFB) was compared to traditional methanol-limited fed-batch technique (MLFB) for the production of recombinant Thai Rosewood β-glucosidase with Pichia pastoris. The degree of energy limitation, expressed as th