Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Megumu, Mabuchi"'
Autor:
Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robb
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-16 (2022)
Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).
Externí odkaz:
https://doaj.org/article/2f051ab7df9a4bb69aca89664afee82f
Autor:
Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to
Externí odkaz:
https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
Publikováno v:
Nucleic Acids Research. 51:3903-3917
The RNA-guided Cas9 endonuclease from Staphylococcus aureus (SauCas9) can catalyze multiple-turnover reactions whereas Cas9 from Streptococcus pyogenes (SpyCas9) is a single-turnover enzyme. Here we dissect the mechanism of multiple-turnover catalysi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::49cdbc413c35ee49c48309fbfdcac458
https://doi.org/10.1093/nar/gkad233
https://doi.org/10.1093/nar/gkad233
Autor:
Kathleen A. Christie, Jimmy A. Guo, Rachel A. Silverstein, Roman M. Doll, Megumu Mabuchi, Hannah E. Stutzman, Jiecong Lin, Linyuan Ma, Russell T. Walton, Luca Pinello, G. Brett Robb, Benjamin P. Kleinstiver
Publikováno v:
Nature Biotechnology. 41:409-416
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d76210e861acf8181c3af196e0bc046a
https://doi.org/10.1038/s41587-022-01492-y
https://doi.org/10.1038/s41587-022-01492-y
Autor:
Tomas Urbaitis, Giedrius Gasiunas, Joshua K Young, Zhenglin Hou, Sushmitha Paulraj, Egle Godliauskaite, Mantvyda M Juskeviciene, Migle Stitilyte, Monika Jasnauskaite, Megumu Mabuchi, G Brett Robb, Virginijus Siksnys
Publikováno v:
EMBO reports, Hoboken : Wiley, 2022, vol. 23, iss. 12, art. no. e55481, p. [1-15]
Most CRISPR-type V nucleases are stimulated to cleave double-stranded (ds) DNA targets by a T-rich PAM, which restricts their targeting range. Here, we identify and characterize a new family of type V RNA-guided nuclease, Cas12l, that exclusively rec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ad27a91f59b7039c4602433f8e2cbba
https://pubmed.ncbi.nlm.nih.gov/36268581
https://pubmed.ncbi.nlm.nih.gov/36268581
Autor:
Justin Albers, Megumu Mabuchi, Ryan T. Fuchs, Prarthana Mohanraju, Richard van Kranenburg, Jennifer L. Curcuru, Ioannis Mougiakos, G. Brett Robb, John van der Oost
Publikováno v:
CRISPR Journal, 4(1), 82-91
CRISPR J
CRISPR Journal 4 (2021) 1
CRISPR J
CRISPR Journal 4 (2021) 1
The ability of CRISPR-Cas12a nucleases to function reliably in a wide range of species has been key to their rapid adoption as genome engineering tools. However, so far, Cas12a nucleases have been limited for use in organisms with growth temperatures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef649b486c311acffb3f3104cae4722f
https://doi.org/10.1089/crispr.2020.0086
https://doi.org/10.1089/crispr.2020.0086
Autor:
Kathleen A. Christie, Jimmy A. Guo, Rachel A. Silverstein, Roman M. Doll, Megumu Mabuchi, Hannah E. Stutzman, Linyuan Ma, G. Brett Robb, Benjamin P. Kleinstiver
While restriction enzymes (REs) remain the gold-standard for manipulating DNA in vitro, they have notable drawbacks including a dependence on short binding motifs that constrain their ability to cleave DNA substrates. Here we overcome limitations of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90883b1070d6818cc6bf7499320c9c40
https://doi.org/10.1101/2022.01.11.474553
https://doi.org/10.1101/2022.01.11.474553
Autor:
Kathleen A, Christie, Jimmy A, Guo, Rachel A, Silverstein, Roman M, Doll, Megumu, Mabuchi, Hannah E, Stutzman, Jiecong, Lin, Linyuan, Ma, Russell T, Walton, Luca, Pinello, G Brett, Robb, Benjamin P, Kleinstiver
Publikováno v:
Nature biotechnology.
Methods for in vitro DNA cleavage and molecular cloning remain unable to precisely cleave DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific motifs, whereas wild-type CRISPR-Cas9 or CRISPR-Cas12 nucleases require
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2bd132efe9eb805fbd83df8e578a7a56
https://pubmed.ncbi.nlm.nih.gov/36203014
https://pubmed.ncbi.nlm.nih.gov/36203014
Autor:
Xinyi He, Megumu Mabuchi, Thomas Lutz, Honorata Czapinska, Alyssa Copelas, Alexey Fomenkov, Kiersten Flodman, Matthias Bochtler, Shuang-yong Xu
Publikováno v:
Nucleic Acids Research
Modification dependent restriction endonucleases (MDREs) often have separate catalytic and modification dependent domains. We systematically looked for previously uncharacterized fusion proteins featuring a PUA or DUF3427 domain and HNH or PD-(D/E)XK
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5b425ed6e66776de0da22d7b3139f64
https://doi.org/10.1093/nar/gkz755
https://doi.org/10.1093/nar/gkz755
Autor:
Ryan T, Fuchs, Jennifer L, Curcuru, Megumu, Mabuchi, Audrey, Noireterre, Peter R, Weigele, Zhiyi, Sun, G Brett, Robb
Publikováno v:
Communications biology. 5(1)
CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs have been repurposed for genome editing in non-native organisms by reprogramming
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d52de699594fead92ca25de292f5a59c
https://pubmed.ncbi.nlm.nih.gov/35388146
https://pubmed.ncbi.nlm.nih.gov/35388146