Zobrazeno 1 - 10
of 56
pro vyhledávání: '"Megan N, McClean"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103002- (2024)
Summary: Gene promoters filter transcription factor (TF) localization dynamics and changes in the DNA binding affinity of TFs. Here, we present a protocol to probe how promoters decode TF dynamics in Saccharomyces cerevisiae by combining optogenetic
Externí odkaz:
https://doaj.org/article/1f0884542082479f94670322667b7c7c
Publikováno v:
mBio, Vol 15, Iss 8 (2024)
ABSTRACT The purine nucleotides ATP and GTP are made from the common precursor inosine monophosphate (IMP). Maintaining the correct balance of these nucleotides for optimal cell growth is controlled in part by the enzyme IMP dehydrogenase (IMPDH), wh
Externí odkaz:
https://doaj.org/article/385c13969c524aac95770a1da8ccd8fa
Autor:
Kevin R. Stindt, Megan N. McClean
Publikováno v:
mSystems, Vol 9, Iss 6 (2024)
ABSTRACT The ability to modify and control natural and engineered microbiomes is essential for biotechnology and biomedicine. Fungi are critical members of most microbiomes, yet technology for modifying the fungal members of a microbiome has lagged f
Externí odkaz:
https://doaj.org/article/9c4c197d316c41aca3ad985a3cfcb763
Autor:
Kieran Sweeney, Megan N. McClean
Publikováno v:
Cell Reports, Vol 42, Iss 5, Pp 112426- (2023)
Summary: Environmental information may be encoded in the temporal dynamics of transcription factor (TF) activation and subsequently decoded by gene promoters to enact stimulus-specific gene expression programs. Previous studies of this behavior focus
Externí odkaz:
https://doaj.org/article/b8eb79d1fcee48a08d1b97f699e8391e
Publikováno v:
eLife, Vol 11 (2022)
Stress defense and cell growth are inversely related in bulk culture analyses; however, these studies miss substantial cell-to-cell heterogeneity, thus obscuring true phenotypic relationships. Here, we devised a microfluidics system to characterize m
Externí odkaz:
https://doaj.org/article/2dd2e93fa21a4ea383f1ecfdd4dffd62
Publikováno v:
BioTechniques, Vol 69, Iss 4, Pp 313-316 (2020)
Optogenetic systems use light to precisely control and investigate cellular processes. Until recently, there had been few instruments available for applying controlled light doses to cultures of cells. The optoPlate, a programmable array of 192 LEDs,
Externí odkaz:
https://doaj.org/article/492b7e0202d545788f16576d9d9311c8
Publikováno v:
APL Bioengineering, Vol 5, Iss 4, Pp 046101-046101-11 (2021)
Utilizing microfluidics to mimic the dynamic temporal changes of growth factor and cytokine concentrations in vivo has greatly increased our understanding of how signal transduction pathways are structured to encode extracellular stimuli. To date, th
Externí odkaz:
https://doaj.org/article/6e489f434f554c78a07a72c7492e983b
Autor:
Neydis Moreno Morales, Michael T. Patel, Cameron J. Stewart, Kieran Sweeney, Megan N. McClean
Publikováno v:
mSphere, Vol 6, Iss 4 (2021)
Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities. In order to better understand the role of these interactions in g
Externí odkaz:
https://doaj.org/article/87e07a7cbe3249a1a20d059997c75dc5
Autor:
Kieran Sweeney, Neydis Moreno Morales, Zachary Burmeister, Amit J. Nimunkar, Megan N. McClean
Publikováno v:
MethodsX, Vol 6, Iss , Pp 1480-1488 (2019)
Optogenetic systems use genetically-encoded light-sensitive proteins to control and study cellular processes. As the number and quality of these systems grows, there is an increasing need for user-friendly and flexible hardware to provide programmed
Externí odkaz:
https://doaj.org/article/7ceefc6842824fc9af4e4be8db4e5b67
Publikováno v:
The FEBS Journal. 290:2097-2114
The yeast mitogen-activated protein kinase pathways serve as a model system for understanding how network interactions affect the way in which cells coordinate the response to multiple signals. We have quantitatively compared two yeast strain backgro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ac11ec25879b2bf04874ba2bbf4720f
https://doi.org/10.1111/febs.16689
https://doi.org/10.1111/febs.16689