Výsledky vyhledávání - "Megan E. Pask"

Akademický článek

Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings

Autor: Christia M. Victoriano, Megan E. Pask, Nicole A. Malofsky, Adam Seegmiller, Steve Simmons, Jonathan E. Schmitz, Frederick R. Haselton, Nicholas M. Adams

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)

Abstract PCR-based diagnostics generally require nucleic acid extraction from patient specimens prior to amplification. As highlighted early in the COVID-19 pandemic, extraction steps may be difficult to scale during times of massive demand and limit

Externí odkaz: https://doaj.org/article/de69f5555a644c808ef462f0d7e32cc4

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Magnetic Bead Processing Enables Sensitive Ligation-Based Detection of HIV Drug Resistance Mutations

Autor: Dalton J. Nelson, Mindy Leelawong, Megan E. Pask, C. William Wester, Muktar H. Aliyu, Frederick R. Haselton

Publikováno v: Analytical chemistry. 94(5)

HIV develops single nucleotide polymorphisms (SNPs), some of which lead to drug resistance mutations (DRMs) that prevent therapeutic viral suppression. Genomic sequencing enables healthcare professionals to select effective combination antiretroviral

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22219887e6dbabbe77c0b663871fd160
https://pubmed.ncbi.nlm.nih.gov/35077642

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Low-Resource Nucleic Acid Extraction Method Enabled by High-Gradient Magnetic Separation

Autor: Anna E. Wolfe, Nicholas M. Adams, Patricia K. Russ, Cassandra Wessely, Megan E. Pask, Mindy Leelawong, Kelly A. Richardson, Frederick R. Haselton, Stephanie I. Pearlman

Publikováno v: ACS Applied Materials & Interfaces

Nucleic acid-based diagnostic tests often require isolation and concentration of nucleic acids from biological samples. Commercial purification kits are difficult to use in low-resource settings because of their cost and insufficient laboratory infra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a4bcb3a49ae8ead3a590c94d9bb02ad
https://pubmed.ncbi.nlm.nih.gov/32039572

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Design and use of mouse control DNA for DNA biomarker extraction and PCR detection from urine: Application for transrenal Mycobacterium tuberculosis DNA detection

Autor: April C. Pettit, Andrew J.M. Nel, David W. Wright, Carlos Zamudio, Frederick R. Haselton, Mark L. Baglia, Megan E. Pask, Keersten M. Ricks, Keertan Dheda, Hali Bordelon, Francesca Solinas, Patricia K. Russ, Tatiana Cáceres, Philip A. Short, Jonathan Michael Blackburn

Urine samples are increasingly used for diagnosing infections including Escherichia coli, Ebola virus, and Zika virus. However, extraction and concentration of nucleic acid biomarkers from urine is necessary for many molecular detection strategies su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c7c930098e0834861b260dcd4529acd
https://hdl.handle.net/20.500.12866/4631

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Translation of Biomedical Prevention Strategies for HIV

Autor: Holly Cassell, Philip J. Ciampa, José A. Tique, Megan E. Pask, Carolyn M. Audet, Sten H. Vermund

Publikováno v: JAIDS Journal of Acquired Immune Deficiency Syndromes. 63:S12-S25

Early achievements in biomedical approaches for HIV prevention included physical barriers (condoms), clean injection equipment (both for medical use and for injection drug users), blood and blood product safety, and prevention of mother to child tran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abd157937d92bda76f58d480bcda6033
https://doi.org/10.1097/qai.0b013e31829202a2

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