Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Megan D. Schertzer"'
Autor:
Aki K. Braceros, Megan D. Schertzer, Arina Omer, Jackson B. Trotman, Eric S. Davis, Jill M. Dowen, Douglas H. Phanstiel, Erez Lieberman Aiden, J. Mauro Calabrese
Publikováno v:
Cell Reports, Vol 42, Iss 7, Pp 112803- (2023)
Summary: During mouse embryogenesis, expression of the long non-coding RNA (lncRNA) Airn leads to gene repression and recruitment of Polycomb repressive complexes (PRCs) to varying extents over a 15-Mb domain. The mechanisms remain unclear. Using hig
Externí odkaz:
https://doaj.org/article/a662b708f3d1487695b89dec71f24f05
Autor:
Aki K. Braceros, Megan D. Schertzer, Arina Omer, Jackson B. Trotman, Eric S. Davis, Jill M. Dowen, Douglas H. Phanstiel, Erez Lieberman Aiden, J. Mauro Calabrese
During mouse embryogenesis, expression of the lncRNAAirninduces gene silencing and recruits Polycomb Repressive Complexes (PRCs) to varying extents over a 15 megabase domain. The mechanisms remain unclear. Using high-resolution approaches, we show in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f9add6ef8dc88740d5119616159e78ca
https://doi.org/10.1101/2022.12.20.521198
https://doi.org/10.1101/2022.12.20.521198
Autor:
David M Lee, Dale O. Cowley, Megan D. Schertzer, Kaoru Inoue, J. Mauro Calabrese, Rachel E Cherney, Jackson B. Trotman, Steven R. Bischoff
Publikováno v:
Nucleic Acids Research
Xist requires Repeat-A, a protein-binding module in its first two kilobases (2kb), to repress transcription. We report that when expressed as a standalone transcript in mouse embryonic stem cells (ESCs), the first 2kb of Xist (Xist-2kb) does not indu
Publikováno v:
Bio Protoc
Long noncoding RNAs (lncRNAs) play essential roles in normal physiology and in disease but their mechanisms of action can be challenging to identify. For mechanistic studies, it is often useful to know a lncRNA's intracellular abundance, i.e., approx
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aff31b5af2a617d2982b84d8701a2cae
https://europepmc.org/articles/PMC7668557/
https://europepmc.org/articles/PMC7668557/
Autor:
Megan D. Schertzer, Dale O. Cowley, Steven R. Bischoff, David M Lee, Kaoru Inoue, J. Mauro Calabrese, Jackson B. Trotman, Rachel E Cherney, Susan O Kim
Publikováno v:
Nucleic Acids Research
The Xist lncRNA requires Repeat A, a conserved RNA element located in its 5′ end, to induce gene silencing during X-chromosome inactivation. Intriguingly, Repeat A is also required for the production of Xist. While silencing by Repeat A requires th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82261efe5cfdda60ca7a2cb36700cee8
https://doi.org/10.1101/2020.05.13.090506
https://doi.org/10.1101/2020.05.13.090506
Autor:
Megan D. Schertzer, Allison R Baker, Daniel Sprague, Qidi Chen, Jessime M. Kirk, Peter J. Mucha, Kevin M. Weeks, David W Collins, J. Mauro Calabrese, Joshua Wooten, Matthew J. Smola, Kaoru Inoue, David M Lee, Shuo Wang, Christopher R Horning, Susan O Kim
Publikováno v:
Nature genetics
The functions of most long non-coding RNAs (lncRNAs) are unknown. In contrast to proteins, lncRNAs with similar functions often lack linear sequence homology; thus, the identification of function in one lncRNA rarely informs the identification of fun
Autor:
Keean C.A. Braceros, J. Mauro Calabrese, Susan O Kim, Emma R Hinkle, Megan D. Schertzer, Ryan M. Murphy, David M Lee, Eva C.M. Vitucci, Eliza Thulson
Publikováno v:
RNA
We describe the development and application of a novel series of vectors that facilitate CRISPR-Cas9-mediated genome editing in mammalian cells, which we call CRISPR-Bac. CRISPR-Bac leverages the piggyBac transposon to randomly insert CRISPR-Cas9 com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a02263b5dddb9db1d15004daac7d30d
https://doi.org/10.1101/448803
https://doi.org/10.1101/448803
Autor:
Megan D. Schertzer, Gabriela Salazar, Dale O. Cowley, J. Mauro Calabrese, Keean C.A. Braceros, Jill M. Dowen, Rachel E Cherney, Mark J. Zylka, David M Lee, Pablo Ariel, Joshua Starmer, Terry Magnuson, Megan Justice, Steven R. Bischoff
Publikováno v:
Mol Cell
Long noncoding RNAs (lncRNAs) cause Polycomb repressive complexes (PRCs) to spread over broad regions of the mammalian genome. We report that in mouse trophoblast stem cells, the Airn and Kcnq1ot1 lncRNAs induce PRC-dependent chromatin modifications
Publikováno v:
G3: Genes|Genomes|Genetics
Several hundred mammalian genes are expressed preferentially from one parental allele as the result of a process called genomic imprinting. Genomic imprinting is prevalent in extra-embryonic tissue, where it plays an essential role during development