An automated cell-counting algorithm for fluorescently-stained cells in migration assays

Autor: Novielli Nicole M, Medeiros Philip J, Al-Khazraji Baraa K, Jackson Dwayne N

Publikováno v: Biological Procedures Online, Vol 13, Iss 1, p 9 (2011)

Abstract A cell-counting algorithm, developed in Matlab®, was created to efficiently count migrated fluorescently-stained cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images were acquired

Externí odkaz: https://doaj.org/article/0eea90d237264db6a2ecaf4619228a87

A murine model of diarrhea, growth impairment and metabolic disturbances with Shigella flexneri infection and the role of zinc deficiency.

Autor: Q S Medeiros PH; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA.; Institute of Biomedicine, Federal University of Ceara , Fortaleza , Brazil., Ledwaba SE; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA., Bolick DT; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA., Giallourou N; Division of Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , London , UK., Yum LK; Department of Microbiology, Immunology and Cancer Biology, University of Virginia , Charlottesville , USA., Costa DVS; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA.; Institute of Biomedicine, Federal University of Ceara , Fortaleza , Brazil., Oriá RB; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA.; Institute of Biomedicine, Federal University of Ceara , Fortaleza , Brazil., Barry EM; Center for Vaccine Development, University of Maryland , Baltimore , USA., Swann JR; Division of Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College London , London , UK., Lima AÂM; Institute of Biomedicine, Federal University of Ceara , Fortaleza , Brazil., Agaisse H; Department of Microbiology, Immunology and Cancer Biology, University of Virginia , Charlottesville , USA., Guerrant RL; Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia , Charlottesville , USA.

Publikováno v: Gut microbes [Gut Microbes] 2019; Vol. 10 (5), pp. 615-630. Date of Electronic Publication: 2019 Feb 03.

Prevalence and virulence gene profiling of enteroaggregative Escherichia coli in malnourished and nourished Brazilian children.

Autor: Havt A; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil. Electronic address: ahavtbinda@gmail.com., Lima IF; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Medeiros PH; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Clementino MA; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Santos AK; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Amaral MS; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Veras HN; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Prata MM; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Lima NL; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Di Moura A; Institute for the Promotion of Nutrition and Human Development, 15 Professor Carlos Lobo, 60281-740, Fortaleza, Ceará, Brazil., Leite ÁM; Institute for the Promotion of Nutrition and Human Development, 15 Professor Carlos Lobo, 60281-740, Fortaleza, Ceará, Brazil., Soares AM; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Filho JQ; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil., Houpt ER; Center for Global Health & Division of Infectious Diseases and International Health and Department of Pediatrics, University of Virginia, 1400 W Main Street, 22908-1379, Charlottesville, VA, USA., Nataro JP; Center for Global Health & Division of Infectious Diseases and International Health and Department of Pediatrics, University of Virginia, 1400 W Main Street, 22908-1379, Charlottesville, VA, USA., Guerrant RL; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil; Center for Global Health & Division of Infectious Diseases and International Health and Department of Pediatrics, University of Virginia, 1400 W Main Street, 22908-1379, Charlottesville, VA, USA., Lima AA; Institute of Biomedicine for Brazilian Semiarid, Federal University of Ceará, 1315 Coronel Nunes de Melo, 60430-270, Fortaleza, Brazil; Center for Global Health & Division of Infectious Diseases and International Health and Department of Pediatrics, University of Virginia, 1400 W Main Street, 22908-1379, Charlottesville, VA, USA.

Publikováno v: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2017 Oct; Vol. 89 (2), pp. 98-105. Date of Electronic Publication: 2017 Jul 04.

Biomarkers of Environmental Enteropathy, Inflammation, Stunting, and Impaired Growth in Children in Northeast Brazil.

Autor: Guerrant RL; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Leite AM; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., Pinkerton R; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Medeiros PH; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., Cavalcante PA; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., DeBoer M; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Kosek M; Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States of America., Duggan C; Division of Gastroenterology at Boston Children's Hospital, Harvard University, Boston, MA, United States of America., Gewirtz A; Institute for Biomedical Sciences in the Center for Inflammation, Immunity and Infection at Georgia State University, Atlanta, GA, United States of America., Kagan JC; Division of Gastroenterology at Boston Children's Hospital, Harvard University, Boston, MA, United States of America., Gauthier AE; Division of Gastroenterology at Boston Children's Hospital, Harvard University, Boston, MA, United States of America., Swann J; Imperial College, London, United Kingdom., Mayneris-Perxachs J; Imperial College, London, United Kingdom., Bolick DT; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Maier EA; Cincinnati Children's Hospital, Cincinnati, OH, United States of America., Guedes MM; Cincinnati Children's Hospital, Cincinnati, OH, United States of America., Moore SR; Cincinnati Children's Hospital, Cincinnati, OH, United States of America., Petri WA; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Havt A; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., Lima IF; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., Prata MM; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil., Michaleckyj JC; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Scharf RJ; University of Virginia School of Medicine (Division of Infectious Diseases and International Health, Department of Medicine, Department of Pediatrics and Center for Global Health), Charlottesville, VA, United States of America., Sturgeon C; Mucosal Immunology and Biology Research Center and Division of Pediatric Gastroenterology and Nutrition at Massachusetts General Hospital for Children, Harvard University, Boston, MA, United States of America., Fasano A; Mucosal Immunology and Biology Research Center and Division of Pediatric Gastroenterology and Nutrition at Massachusetts General Hospital for Children, Harvard University, Boston, MA, United States of America., Lima AA; Clinical Research Unit, Federal University of Ceara, Fortaleza, Brazil.

Publikováno v: PloS one [PLoS One] 2016 Sep 30; Vol. 11 (9), pp. e0158772. Date of Electronic Publication: 2016 Sep 30 (Print Publication: 2016).

Correction: Defined Nutrient Diets Alter Susceptibility to Clostridium difficile Associated Disease in a Murine Model.

Autor: Moore JH, Pinheiro CC, Zaenker EI, Bolick DT, Kolling GL, van Opstal E, Noronha FJ, De Medeiros PH, Rodriguez RS, Lima AA, Guerrant RL, Warren CA

Publikováno v: PloS one [PLoS One] 2015 Sep 15; Vol. 10 (9), pp. e0137037. Date of Electronic Publication: 2015 Sep 15 (Print Publication: 2015).