Why the Energy Landscape of Barnase Is Hierarchical

Autor: Maya J. Pandya, Stefanie Schiffers, Andrea M. Hounslow, Nicola J. Baxter, Mike P. Williamson

Publikováno v: Frontiers in Molecular Biosciences, Vol 5 (2018)

We have used NMR and computational methods to characterize the dynamics of the ribonuclease barnase over a wide range of timescales in free and inhibitor-bound states. Using temperature- and denaturant-dependent measurements of chemical shift, we sho

Externí odkaz: https://doaj.org/article/36e10a5ca2514b99be6ec5e2bbc29727

Pressure-Dependent Structure Changes in Barnase on Ligand Binding Reveal Intermediate Rate Fluctuations

Autor: Maya J. Pandya, Kazuyuki Akasaka, Ryo Kitahara, David J. Wilton, Michael P. Williamson

Publikováno v: Biophysical Journal. 97(5):1482-1490

In this work we measured 1H NMR chemical shifts for the ribonuclease barnase at pressures from 3 MPa to 200 MPa, both free and bound to d(CGAC). Shift changes with pressure were used as restraints to determine the change in structure with pressure. F

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::915a56bf6cf6b7bfe45f33bf49651beb

Engineering Increased Stability into Self-Assembled Protein Fibers

Autor: Wayne R. Edwards, Andrew M. Smith, Maya J. Pandya, Eleanor F. Banwell, Derek N. Woolfson

Publikováno v: Smith, A, BANWELL, EF, EDWARDS, WR, PANDYA, MJ & WOOLFSON, DN 2006, ' Engineering increased stability into self-assembled protein fibers ', Advanced Functional Materials, vol. 16, no. 8, pp. 1022-1030 . https://doi.org/10.1002/adfm.200500568

Two stages in the rational redesign of a peptide-based, self-assembling fiber (SAF) are described. The SAF system comprises two peptides designed to form an offset a-helical coiled-coil heterodimer. The "sticky-ends" are complementary and promote lon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d5f44f879282482f96013059a28a1a1
https://doi.org/10.1002/adfm.200500568

Sticky-End Assembly of a Designed Peptide Fiber Provides Insight into Protein Fibrillogenesis

Autor: Gillian M. Spooner, Derek N. Woolfson, Julian R. Thorpe, Margaret Sunde, Maya J. Pandya, and Alison Rodger

Publikováno v: Biochemistry. 39:8728-8734

Coiled-coil motifs provide simple systems for studying molecular self-assembly. We designed two 28-residue peptides to assemble into an extended coiled-coil fiber. Complementary interactions in the core and flanking ion-pairs were used to direct stag

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb3e364edb62582ab7003297b7f5c7bf
https://doi.org/10.1021/bi000246g

Direct Kinetic Evidence for Folding via a Highly Compact, Misfolded State

Autor: Peter R. Shewry, Phil B. Williams, Maya J. Pandya, Anthony R. Clarke, Christopher E. Dempsey

Publikováno v: University of Bristol-PURE

The 2 S seed storage protein, sunflower albumin 8 (SFA-8), contains an unusually high proportion of hydrophobic residues including 16 methionines (some of which may form a surface hydrophobic patch) in a disulfide cross-linked, alpha-helical structur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::359de6d36e333396ac2d433caf2147de
https://doi.org/10.1074/jbc.274.38.26828