Human FSH Glycoform α-Subunit Asparagine ⁵² Glycans: Major Glycan Structural Consistency, Minor Glycan Variation in Abundance.

Autor: Butnev VY; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., May JV; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., Brown AR; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., Sharma T; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., Butnev VY; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., White WK; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., Harvey DJ; Department of Biochemistry, University of Oxford, Oxford, United Kingdom., Bousfield GR; Department of Biological Sciences, Wichita State University, Wichita, KS, United States.

Publikováno v: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2022 Oct 18; Vol. 13, pp. 767661. Date of Electronic Publication: 2022 Oct 18 (Print Publication: 2022).

Bioactivity of recombinant hFSH glycosylation variants in primary cultures of porcine granulosa cells.

Autor: Liang A; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China., Plewes MR; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Ave, Omaha, NE, 68105, USA., Hua G; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China., Hou X; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA., Blum HR; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA., Przygrodzka E; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA., George JW; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Ave, Omaha, NE, 68105, USA., Clark KL; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Ave, Omaha, NE, 68105, USA., Bousfield GR; Department of Biological Sciences, Wichita State University, Wichita, KS, 67260, USA., Butnev VY; Department of Biological Sciences, Wichita State University, Wichita, KS, 67260, USA., May JV; Department of Biological Sciences, Wichita State University, Wichita, KS, 67260, USA., Davis JS; Olson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA; Veterans Affairs Nebraska Western Iowa Health Care System, 4101 Woolworth Ave, Omaha, NE, 68105, USA. Electronic address: jsdavis@unmc.edu.

Publikováno v: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2020 Aug 20; Vol. 514, pp. 110911. Date of Electronic Publication: 2020 Jun 15.

In Vivo and In Vitro Impact of Carbohydrate Variation on Human Follicle-Stimulating Hormone Function.

Autor: Bousfield GR; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., May JV; Department of Biological Sciences, Wichita State University, Wichita, KS, United States., Davis JS; Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, United States.; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, United States.; Nebraska-Western Iowa Health Care System, Omaha, NE, United States., Dias JA; Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY, United States., Kumar TR; Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, Aurora, CO, United States.

Publikováno v: Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2018 May 09; Vol. 9, pp. 216. Date of Electronic Publication: 2018 May 09 (Print Publication: 2018).

Evaluation of in vivo bioactivities of recombinant hypo- (FSH ^21/18 ) and fully- (FSH ²⁴ ) glycosylated human FSH glycoforms in Fshb null mice.

Autor: Wang H; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA., May J; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA., Butnev V; Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA., Shuai B; Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA., May JV; Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA., Bousfield GR; Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA., Kumar TR; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA; Center for Reproductive Sciences, Institute for Reproductive Health and Regenerative Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA; Department of Neurosurgery, University of Kansas Medical Center, Kansas City, KS 66160, USA; Division of Reproductive Sciences, Department of Obstetrics & Gynecology, University of Colorado Denver-Anschutz Medical Campus, Aurora, CO 80045, USA. Electronic address: raj.kumar@ucdenver.edu.

Publikováno v: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2016 Dec 05; Vol. 437, pp. 224-236. Date of Electronic Publication: 2016 Aug 22.

Hypoglycosylated hFSH Has Greater Bioactivity Than Fully Glycosylated Recombinant hFSH in Human Granulosa Cells.

Autor: Jiang C; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., Hou X; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., Wang C; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., May JV; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., Butnev VY; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., Bousfield GR; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198., Davis JS; The Olson Center for Women's Health, Department of Obstetrics and Gynecology (C.J., X.H., C.W., J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198; Nebraska-Northwest Iowa VA Medical Center (X.H., J.S.D.), Omaha, Nebraska 68105; Department of Biological Sciences (J.V.M., V.Y.B., G.R.B.), Wichita State University, Wichita, Kansas 67260; and Department of Biochemistry and Molecular Biology (J.S.D.), University of Nebraska Medical Center, Omaha, Nebraska 68198.

Publikováno v: The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2015 Jun; Vol. 100 (6), pp. E852-60. Date of Electronic Publication: 2015 Apr 27.