Spacer capture and integration by a type I-F Cas1–Cas2-3 CRISPR adaptation complex

Autor: Fagerlund, Robert D., Wilkinson, Max E., Klykov, Oleg, Barendregt, Arjan, Pearce, F. Grant, Kieper, Sebastian N., Maxwell, Howard W. R., Capolupo, Angela, Heck, Albert J. R., Krause, Kurt L., Bostina, Mihnea, Scheltema, Richard A., Staals, Raymond H. J., Fineran, Peter C.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 2017 Jun . 114(26), E5122-E5128.

Externí odkaz: https://www.jstor.org/stable/26485015

Reconstitution of CRISPR adaptation in vitro and its detection by PCR.

Autor: Fagerlund RD; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. Electronic address: robert.fagerlund@otago.ac.nz., Ferguson TJ; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Maxwell HWR; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Opel-Reading HK; Department of Biochemistry, University of Otago, Dunedin, New Zealand., Krause KL; Department of Biochemistry, University of Otago, Dunedin, New Zealand., Fineran PC; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand. Electronic address: peter.fineran@otago.ac.nz.

Publikováno v: Methods in enzymology [Methods Enzymol] 2019; Vol. 616, pp. 411-433. Date of Electronic Publication: 2019 Jan 12.