Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Max W. Shen"'
Autor:
Jonathan C. Chen, Jonathan P. Chen, Max W. Shen, Michael Wornow, Minwoo Bae, Wei-Hsi Yeh, Alvin Hsu, David R. Liu
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
In vitro library screening is a powerful approach to identify functional biopolymers, but only covers a fraction of possible sequences. Here, the authors use experimental in vitro selection results to train a conditional variational autoencoder machi
Externí odkaz:
https://doaj.org/article/502e0dcaf2ca4731b191cf430212bb7e
Autor:
Minja Velimirovic, Larissa C. Zanetti, Max W. Shen, James D. Fife, Lin Lin, Minsun Cha, Ersin Akinci, Danielle Barnum, Tian Yu, Richard I. Sherwood
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. Here the authors present PepSEq, a high-throughput method for screening a large library of peptides that influence prime editing efficiency.
Externí odkaz:
https://doaj.org/article/d055f5b29f2642d4ace3d63f203574ad
Autor:
Heysol C. Bermudez-Cabrera, Sannie Culbertson, Sammy Barkal, Benjamin Holmes, Max W. Shen, Sophia Zhang, David K. Gifford, Richard I. Sherwood
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
The mutational outcome of CRISPR-Cas9 editing can be both predictable and targeted. Here the authors show that ATM inhibitor KU-60019 increases 1 bp insertions at the targeted locus.
Externí odkaz:
https://doaj.org/article/77675116590e4b8aa7e1d10c6a4309b0
Autor:
Jonathan Y. Hsu, Julian Grünewald, Regan Szalay, Justine Shih, Andrew V. Anzalone, Kin Chung Lam, Max W. Shen, Karl Petri, David R. Liu, J. Keith Joung, Luca Pinello
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Prime editing guide RNA design is more complex than for standard CRISPR-based nucleases or base editors. Here the authors present PrimeDesign and PrimeVar for the rapid and simplified design of pegRNA and ngRNA combinations.
Externí odkaz:
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
Autor:
Mandana Arbab, Zaneta Matuszek, Kaitlyn M. Kray, Ailing Du, Gregory A. Newby, Anton J. Blatnik, Aditya Raguram, Michelle F. Richter, Kevin T. Zhao, Jonathan M. Levy, Max W. Shen, W. David Arnold, Dan Wang, Jun Xie, Guangping Gao, Arthur H. M. Burghes, David R. Liu
Publikováno v:
Science. 380
Spinal muscular atrophy (SMA), the leading genetic cause of infant mortality, arises from survival motor neuron (SMN) protein insufficiency resulting from SMN1 loss. Approved therapies circumvent endogenous SMN regulation and require repeated dosing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da790fc009f5f996838b159db859f45f
https://doi.org/10.1126/science.adg6518
https://doi.org/10.1126/science.adg6518
Autor:
Luke W. Koblan, Britt Adamson, Albert Xu, Mandana Arbab, Tyler A Sisley, Jordan L. Doman, Dian Yang, Joseph M. Replogle, David R. Liu, Andrew V. Anzalone, Jeffrey A. Hussmann, Max W. Shen, Gregory A. Newby, Beverly Mok, Jonathan S. Weissman
Publikováno v:
Nature Biotechnology. 39:1414-1425
Programmable C•G-to-G•C base editors (CGBEs) have broad scientific and therapeutic potential, but their editing outcomes have proved difficult to predict and their editing efficiency and product purity are often low. We describe a suite of engine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::794631ca2dbb8219581743d613b4016c
https://doi.org/10.1038/s41587-021-00938-z
https://doi.org/10.1038/s41587-021-00938-z
Autor:
Luca Pinello, Max W. Shen, Regan Szalay, Julian Grünewald, Jonathan Y. Hsu, J. Keith Joung, Andrew V. Anzalone, Justine Shih, David R. Liu, Kin Chung Lam, Karl Petri
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Nature Communications
Nature Communications
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ef65e9d4e85e74b4a85709efb6e1dc2
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
Autor:
Max W. Shen, Mandana Arbab, Peyton B. Randolph, Tony P. Huang, Gregory A. Newby, Zaneta Matuszek, Tina Wang, Holly A. Rees, David R. Liu, Shannon M. Miller
Publikováno v:
Nature biotechnology
The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing Gs. Here, we report the evolution of three new SpCas9 variants that collectively re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4854f8fda8cc240d332cdf315fc1e91
https://doi.org/10.1038/s41587-020-0412-8
https://doi.org/10.1038/s41587-020-0412-8
Autor:
Ersin Akinci, James D. Fife, Richard I. Sherwood, Minja Velimirovic, Minsun Cha, Max W. Shen, Tian Yu, Larissa C. Zanetti, Danielle Barnum, Lin Lin
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, PepSEq, to measure how fusion of 12,000 85-amino acid peptides derived from human DNA repair-related proteins inf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e37f7be920a823f26014b3e2c1f0384
https://doi.org/10.1101/2021.09.22.461415
https://doi.org/10.1101/2021.09.22.461415
Autor:
Richard I. Sherwood, Sammy A Barkal, Sannie J. Culbertson, Sophia Zhang, Heysol C Bermudez-Cabrera, Max W. Shen, Benjamin Holmes, David K. Gifford
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Nature Communications
Nature Communications
Mutational outcomes following CRISPR-Cas9-nuclease cutting in mammalian cells have recently been shown to be predictable and, in certain cases, skewed toward single genotypes. However, the ability to control these outcomes remains limited, especially
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e49ed019225d0508ad5181b877277d0
https://doi.org/10.1038/s41467-021-25415-8
https://doi.org/10.1038/s41467-021-25415-8