Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Max G. Schubert"'
Autor:
Annesha Sengupta, Anindita Bandyopadhyay, Debolina Sarkar, John I. Hendry, Max G. Schubert, Deng Liu, George M. Church, Costas D. Maranas, Himadri B. Pakrasi
Publikováno v:
mBio, Vol 15, Iss 3 (2024)
ABSTRACT Cyanobacteria are photosynthetic organisms that have garnered significant recognition as potential hosts for sustainable bioproduction. However, their complex regulatory networks pose significant challenges to major metabolic engineering eff
Externí odkaz:
https://doaj.org/article/5967109df3564918935e1b8fda0efbac
Autor:
Annesha Sengupta, Anindita Bandyopadhyay, Max G. Schubert, George M. Church, Himadri B. Pakrasi
Publikováno v:
Microbiology Spectrum, Vol 11, Iss 4 (2023)
ABSTRACT Our planet is sustained by sunlight, the primary energy source made accessible to all life forms by photoautotrophs. Photoautotrophs are equipped with light-harvesting complexes (LHCs) that enable efficient capture of solar energy, particula
Externí odkaz:
https://doaj.org/article/3867fe663711406e8a3f7193f56a1333
Publikováno v:
Materials Today Bio, Vol 19, Iss , Pp 100583- (2023)
Cyanobacteria are ideal candidates to use in developing carbon neutral and carbon negative technologies; they are efficient photosynthesizers and amenable to genetic manipulation. Over the past two decades, researchers have demonstrated that cyanobac
Externí odkaz:
https://doaj.org/article/8b17e2a884d84786983e2537bcf343d3
Autor:
Santi Bhattarai-Kline, Sierra K. Lear, Chloe B. Fishman, Santiago C. Lopez, Elana R. Lockshin, Max G. Schubert, Jeff Nivala, George Church, Seth L. Shipman
Publikováno v:
Nature
Biological processes depend on the differential expression of genes over time, but methods to make physical recordings of these processes are limited. Here we report a molecular system for making time-ordered recordings of transcriptional events into
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a9b20044d7908865f925206ef39adce
https://europepmc.org/articles/PMC9357182/
https://europepmc.org/articles/PMC9357182/
Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells
Autor:
Eriona Hysolli, Michaela A. Jones, Max G. Schubert, Karen Noguera, Daniel J. Mandell, Evan Appleton, Aditya M. Kunjapur, George M. Church, Siddharth Iyer, Michael G. Napolitano
Publikováno v:
Science Advances. 7
Understanding the evolutionary stability and possible context-dependence of biological containment techniques is critical as engineered microbes are increasingly under consideration for applications beyond biomanufacturing. While batch cultures of sy
Autor:
Seth L. Shipman, Timothy K. Lu, Divjot Kaur, Daniel B. Goodman, Fahim Farzadfard, Max G. Schubert, George M. Church, Timothy M. Wannier
Publikováno v:
Proc Natl Acad Sci U S A
Creating and characterizing individual genetic variants remains limited in scale, compared to the tremendous variation both existing in nature and envisioned by genome engineers. Here we introduce retron library recombineering (RLR), a methodology fo
Autor:
Ákos Nyerges, Michaela A. Jones, Peter N. Ciaccia, Andrew D. Ellington, Csaba Pál, Kamyab Javanmardi, George M. Church, Farren J. Isaacs, Aditya M. Kunjapur, Gabriel T. Filsinger, Timothy M. Wannier, Max G. Schubert
Publikováno v:
Nat Rev Methods Primers
Nature Reviews Methods Primers
Nature Reviews Methods Primers
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved geno
Autor:
Seth L. Shipman, Timothy M. Wannier, Daniel B. Goodman, Timothy K. Lu, George M. Church, Max G. Schubert, Divjot Kaur, Fahim Farzadfard
Tremendous genetic variation exists in nature, but our ability to create and characterize individual genetic variants remains far more limited in scale. Likewise, engineering proteins and phenotypes requires the introduction of synthetic variants, bu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d0e172c83b480708150f3308a588b0e
https://doi.org/10.1101/2020.03.05.975441
https://doi.org/10.1101/2020.03.05.975441
Autor:
Jessica M, Walter, Max G, Schubert, Stephanie H, Kung, Kristy, Hawkins, Darren M, Platt, Aaron D, Hernday, Tina, Mahatdejkul-Meadows, Wayne, Szeto, Sunil S, Chandran, Jack D, Newman, Andrew A, Horwitz
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2049
CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f
Autor:
Kristy Michelle Hawkins, Darren Platt, Max G. Schubert, Sunil S. Chandran, Andrew A. Horwitz, Jack D. Newman, Tina Mahatdejkul-Meadows, Jessica M. Walter, Stephanie H. Kung, Aaron D. Hernday, Wayne Szeto
Publikováno v:
Methods in Molecular Biology ISBN: 9781493997350
CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ad30205bac9a685c020b058739f269cf
https://doi.org/10.1007/978-1-4939-9736-7_3
https://doi.org/10.1007/978-1-4939-9736-7_3