Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Max Emperle"'
Autor:
Leonie Dossmann, Max Emperle, Michael Dukatz, Alex de Mendoza, Pavel Bashtrykov, Albert Jeltsch
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-12 (2024)
Abstract The DNA methyltransferase DNMT3C appeared as a duplication of the DNMT3B gene in muroids and is required for silencing of young retrotransposons in the male germline. Using specialized assay systems, we investigate the flanking sequence pref
Externí odkaz:
https://doaj.org/article/926964ba06e8461bbcb0aa1dd8e6a35e
Autor:
Linfeng Gao, Max Emperle, Yiran Guo, Sara A. Grimm, Wendan Ren, Sabrina Adam, Hidetaka Uryu, Zhi-Min Zhang, Dongliang Chen, Jiekai Yin, Michael Dukatz, Hiwot Anteneh, Renata Z. Jurkowska, Jiuwei Lu, Yinsheng Wang, Pavel Bashtrykov, Paul A. Wade, Gang Greg Wang, Albert Jeltsch, Jikui Song
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
In mammals, DNA methylation patterns are established by two de novo DNA methyltransferases, DNMT3A and DNMT3B. Here the authors report the crystal structures of DNMT3B in complex with both CpG and CpA DNA, providing insight into the substrate-recogni
Externí odkaz:
https://doaj.org/article/effe2dab19bf4176b1b1739202fca190
Publikováno v:
Methods and Protocols, Vol 4, Iss 1, p 3 (2020)
Mal d 1 is the primary apple allergen in northern Europe. To explain the differences in the allergenicity of apple varieties, it is essential to study its properties and interaction with other phytochemicals, which might modulate the allergenic poten
Externí odkaz:
https://doaj.org/article/d67bcf8f5af240e1a01d36a5c1b1ba17
Autor:
Stefan Kunert, Max Emperle, Sabrina Adam, Julia Bräcker, Jens Brockmeyer, Arumugam Rajavelu, Albert Jeltsch
Publikováno v:
Biochimie. 208:66-74
The DNMT3A DNA methyltransferase is an important epigenetic enzyme that is frequently mutated in cancers, particularly in AML. The heterozygous R736H mutation in the FF-interface of the tetrameric enzyme is the second most frequently observed DNMT3A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7180e985e29c45bad22601f7a5dcaac7
https://doi.org/10.1016/j.biochi.2022.12.005
https://doi.org/10.1016/j.biochi.2022.12.005
Autor:
Alexandra Mack, Max Emperle, Philipp Schnee, Sabrina Adam, Jürgen Pleiss, Pavel Bashtrykov, Albert Jeltsch
Publikováno v:
Journal of molecular biology. 434(7)
Somatic R882H DNMT3A mutations occur frequently in AML, but their pathogenic mechanism is unclear. As R882H mutations usually are heterozygous, wildtype (WT) and R882H subunits co-exist in affected cells. R882 is located in the RD interface of DNMT3A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1966ade0953518a04680d3222eb1adb5
https://pubmed.ncbi.nlm.nih.gov/35131259
https://pubmed.ncbi.nlm.nih.gov/35131259
Autor:
Ingrid Tessmer, Hannah S. Heil, Pavel Bashtrykov, Katrin G. Heinze, Albert Jeltsch, Disha M. Bangalore, Stefan Kunert, Sabrina Adam, Max Emperle
Publikováno v:
Nucleic Acids Research
DNMT3A/3L heterotetramers contain two active centers binding CpG sites at 12 bp distance, however their interaction with DNA not containing this feature is unclear. Using randomized substrates, we observed preferential co-methylation of CpG sites wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e9db252b2cec3ab9ea90a62fd37208f
https://pubmed.ncbi.nlm.nih.gov/34289056
https://pubmed.ncbi.nlm.nih.gov/34289056
Autor:
Christoph Plass, Pavel Bashtrykov, Michael Dukatz, Philipp Rathert, Stefan Kunert, Annika Baude, Albert Jeltsch, Sabrina Adam, Max Emperle
Publikováno v:
Nucleic Acids Research
Somatic DNMT3A mutations at R882 are frequently observed in AML patients including the very abundant R882H, but also R882C, R882P and R882S. Using deep enzymology, we show here that DNMT3A-R882H has more than 70-fold altered flanking sequence prefere
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::235bc8911cb79cb85916d6742514bde5
https://doi.org/10.1093/nar/gkz911
https://doi.org/10.1093/nar/gkz911
Autor:
Petra Hajkova, Cristina E. Requena, Peter Sarkies, Michael Dukatz, Albert Jeltsch, Max Emperle
Publikováno v:
Journal of molecular biology
Recently, it has been discovered that different DNA-(cytosine C5)-methyltransferases including DNMT3A generate low levels of 3mC [Rosic et al. (2018), Nat. Genet., 50, 452–459]. This reaction resulted in the co-evolution of DNMTs and ALKB2 DNA repa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1805ee70b255dfb23b64fda7d19d7c7d
https://doi.org/10.1016/j.jmb.2019.06.015
https://doi.org/10.1016/j.jmb.2019.06.015
Autor:
Max Emperle, Jayant K. Rane, Cristina E. Requena, Philipp H. Schiffer, Toni Beltran, Ana R. Gomes, Richard K. Grencis, Petra Hajkova, Murray E. Selkirk, Silvana Rošić, Allison J. Bancroft, Albert Jeltsch, Sarah Linnett, Peter Sarkies, Rachel Amouroux
Publikováno v:
Nature genetics
Nature Genetics
Nature Genetics
Methylation at the 5 position of cytosine in DNA (5meC), is a key epigenetic mark in eukaryotes. Once introduced, 5meC can be maintained through DNA replication due to the activity of “maintenance” DNA methyltransferases.. Despite their ancient o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a104cac6828f786dbaf9a4b1a17e366
https://doi.org/10.1038/s41588-018-0061-8
https://doi.org/10.1038/s41588-018-0061-8
Publikováno v:
Journal of Molecular Biology. 433:167186
DNA interacting enzymes recognize their target sequences embedded in variable flanking sequence context. The influence of flanking sequences on enzymatic activities of DNA methyltransferases (DNMTs) can be systematically studied with “deep enzymolo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72e7d7c90cec4da6fb2dc8817a9dc878
https://doi.org/10.1016/j.jmb.2021.167186
https://doi.org/10.1016/j.jmb.2021.167186