Zobrazeno 1 - 10
of 83
pro vyhledávání: '"Maurice M. Moloney"'
Publikováno v:
Nature Biotechnology. 38:1025-1027
Publikováno v:
Plant cell reports. 17(3)
Hirudin, a blood anticoagulant protein from leeches, and β-glucuronidase were produced in Brassica carinata Braun (Ethiopian mustard) seeds using oleosin as a carrier. Cotyledonary petioles were infected with Agrobacterium strains containing oleosin
Publikováno v:
Plant cell reports. 17(1)
An Arabidopsis oleosin gene including 860 bp of promoter as well as coding sequence was fused to the reporter, β-glucuronidase (GUS). In transient expression assays following particle bombardment, the Arabidopsis gene in both transcriptional and tra
Autor:
Maurice M. Moloney, Jean-Claude Tardif, Elizabeth Wanda Murray, Reid Alexandra, David Busseuil, Cory Nykiforuk, Joseph Boothe, Yin Shen, Eric Rhéaume
Publikováno v:
Plant Biotechnology Journal. 9:250-263
Apolipoprotein AI Milano (ApoAI(Milano) ) was expressed as a fusion protein in transgenic safflower seeds. High levels of expression corresponding to 7 g of ApoAI(Milano) per kilogram of seed have been identified in a line selected for commercializat
Autor:
Steven Szarka, Steven Zaplachinski, Maurice M. Moloney, Cory Nykiforuk, Douglas W. Morck, Joseph Boothe, Elizabeth Wanda Murray, Yin Shen, Philip A. Kuhlman
Publikováno v:
Plant Biotechnology Journal. 8:588-606
The evolution of the seed system provides enormous adaptability to the gymnosperms and angiosperms, because of the properties of dormancy, nutrient storage and seedling vigour. Many of the unique properties of seeds can be exploited in molecular farm
Autor:
Randall J. Weselake, Antoni Banaś, Olga Yurchenko, Maurice M. Moloney, Ulf Stahl, Cory Nykiforuk, Sten Stymne
Publikováno v:
Plant Biotechnology Journal. 7:602-610
The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus was over-expressed in developing seeds of Arabidopsis thaliana. Biochemical analysis of T(2) and T(3) A. thaliana seeds revealed a significant increase in polyunsaturated
Publikováno v:
Biotechnology Advances. 25:148-175
The increasing effect of non-degradable plastic wastes is a growing concern. Polyhydroxyalkanoates (PHAs), macromolecule-polyesters naturally produced by many species of microorganisms, are being considered as a replacement for conventional plastics.
Autor:
Edward C. Yeung, Arturo Lopez-Villalobos, Kim Findlay, Rodrigo M.P. Siloto, Cory Nykiforuk, Maurice M. Moloney
Publikováno v:
The Plant Cell. 18:1961-1974
We investigated the role of the oilbody proteins in developing and germinating Arabidopsis thaliana seeds. Seed oilbodies are simple organelles comprising a matrix of triacylglycerol surrounded by a phospholipid monolayer embedded and covered with un
Publikováno v:
The Plant Journal. 37:461-470
Oleosin protein is targeted to oil bodies via the endoplasmic reticulum (ER) and consists of a lipid-submerged hydrophobic (H) domain that is flanked by cytosolic hydrophilic domains. We investigated the relationship between oleosin ER topology and i
Publikováno v:
Scopus-Elsevier
Expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein. In general, such fusion proteins must be cleaved to release the mature prot