Statistical models discriminating between complex samples measured with microfluidic receptor-cell arrays.

Autor: Ron Wehrens, Margriet Roelse, Maurice Henquet, Marco van Lenthe, Paul W Goedhart, Maarten A Jongsma

Publikováno v: PLoS ONE, Vol 14, Iss 4, p e0214878 (2019)

Data analysis for flow-based in-vitro receptomics array, like a tongue-on-a-chip, is complicated by the relatively large variability within and between arrays, transfected DNA types, spots, and cells within spots. Simply averaging responses of spots

Externí odkaz: https://doaj.org/article/4304e8d6026f4d7e9ab9c30844e6cc7b

Calcium Imaging of GPCR Activation Using Arrays of Reverse Transfected HEK293 Cells in a Microfluidic System

Autor: Marco S. van Lenthe, Robert Hall, Ron Wehrens, Maurice Henquet, Margriet Roelse, Norbert C.A. de Ruijter, Harrie A. Verhoeven, Maarten A. Jongsma, Renger F. Witkamp

Publikováno v: Sensors; Volume 18; Issue 2; Pages: 602
Sensors (Switzerland) 18 (2018) 2
Sensors, Vol 18, Iss 2, p 602 (2018)
Sensors (Switzerland), 18(2)
Sensors (Basel, Switzerland)

Reverse-transfected cell arrays in microfluidic systems have great potential to perform large-scale parallel screening of G protein-coupled receptor (GPCR) activation. Here, we report the preparation of a novel platform using reverse transfection of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33fc3ea1c6fd2ffe7b03c8f3445bb042

Identification of a drimenol synthase and drimenol oxidase from Persicaria hydropiper, involved in the biosynthesis of insect deterrent drimanes

Autor: Marina Varbanova-Herde, Marcel Prins, Harro J. Bouwmeester, Michiel T. J. de Both, Martin De Vos, Maarten A. Jongsma, Maurice C. R. Franssen, Neli Prota, Raymond J. M. Hulzink, Justin J. J. van der Hooft, Maurice Henquet

Publikováno v: The Plant Journal, 90(6), 1052-1063
Plant Journal, 90(6), 1052-1063. Wiley-Blackwell
The Plant Journal 90 (2017) 6

The sesquiterpenoid polygodial, which belongs to the drimane family, has been shown to be an antifeedant for a number of herbivorous insects. It is presumed to be synthesized from farnesyl diphosphate via drimenol, subsequent C‐12 hydroxylation and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29afef398e592c3a9373f8a153588bdb
https://research.wur.nl/en/publications/identification-of-a-drimenol-synthase-and-drimenol-oxidase-from-p

Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody

Autor: Juha-Pekka Pitkänen, Maurice Henquet, Stefan Schillberg, Suvi T. Häkkinen, Tibor Anderlei, Marja-Leena Laukkanen, Dirk Bosch, Richard M. Twyman, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Nicole Raven

Publikováno v: Biotechnology and Bioengineering. 111:336-346

Recombinantpharmaceuticalproteinsexpressed in hairy root cultures can be secreted into the medium to improve product homogeneity and to facilitate purification, although this may result in significant degradation if the protein is inherently unstable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a9934b5a159db9281dfa36f19b0a2528
https://doi.org/10.1002/bit.25113

LEW3, encoding a putative α-1,2-mannosyltransferase (ALG11) in N-linked glycoprotein, plays vital roles in cell-wall biosynthesis and the abiotic stress response in Arabidopsis thaliana

Autor: Dirk Bosch, Xiaozhi Ren, Hairong Zhang, Min Zhang, Maurice Henquet, Zhizhong Gong, Zhizhong Chen, Martine Gonneau, Yi Zhang, Sander van der Krol

Publikováno v: The Plant Journal. 60:983-999

Summary N-linked glycosylation is an essential protein modification that helps protein folding, trafficking and translocation in eukaryotic systems. The initial process for N-linked glycosylation shares a common pathway with assembly of a dolichol-li

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7a4e1bdf6b2a80573af879eb24112241
https://doi.org/10.1111/j.1365-313x.2009.04013.x