Interbase-FRET binding assay for pre-microRNAs

Autor: Mattias Bood, Anna Wypijewska del Nogal, Jesper R. Nilsson, Fredrik Edfeldt, Anders Dahlén, Malin Lemurell, L. Marcus Wilhelmsson, Morten Grøtli

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)

Abstract The aberrant expression of microRNAs (miRs) has been linked to several human diseases. A promising approach for targeting these anomalies is the use of small-molecule inhibitors of miR biogenesis. These inhibitors have the potential to (i) d

Externí odkaz: https://doaj.org/article/cda9c7e7160e4417b694ff5cf0c29da7

Getting DNA and RNA out of the dark with 2CNqA: a bright adenine analogue and interbase FRET donor

Autor: Pauline Pfeiffer, Tom Brown, Jesper R. Nilsson, Anders Dahlén, Mattias Bood, Anders Foller Füchtbauer, Marcus Wilhelmsson, Afaf H. El-Sagheer, Anna Wiktoria Wypijewska Del Nogal, Vinoth Sundar Rajan, Sangamesh Sarangamath, Morten Grøtli, Moa Sandberg Wranne

Publikováno v: Nucleic Acids Research

With the central role of nucleic acids there is a need for development of fluorophores that facilitate the visualization of processes involving nucleic acids without perturbing their natural properties and behaviour. Here, we incorporate a new analog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cd7ab70571c5801ffb216cb97c04675
https://doi.org/10.1093/nar/gkaa525

Interbase FRET in RNA: from A to Z

Autor: Erik Weis, Anders Foller Füchtbauer, Pauline Pfeiffer, Mattias Bood, Jesper R. Nilsson, Anders Dahlén, Marcus Wilhelmsson, Moa Sandberg Wranne, Morten Grøtli

Publikováno v: Nucleic Acids Research

Published by Oxford University Press on behalf of Nucleic Acids Research. Interbase FRET can reveal highly detailed information about distance, orientation and dynamics in nucleic acids, complementing the existing structure and dynamics techniques. W

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::781163d336b5e27287d63ff97b8f4f90
https://doi.org/10.1093/nar/gkz812

Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analogue

Autor: Anders Dahlén, Byeang Hyean Kim, Anita C. Jones, Tom Brown, Moa Sandberg Wranne, Mattias Bood, Anders Foller Füchtbauer, Déborah L M Rupert, Rachel S. Fisher, Morten Grøtli, Fredrik Höök, Sangamesh Sarangamath, Marcus Wilhelmsson, Steven W. Magennis, Jong Jin Ro, Afaf H. El-Sagheer

Publikováno v: Bood, M, Füchtbauer, A F, Wranne, M S, Ro, J J, Sarangamath, S, El-sagheer, A H, Rupert, D L M, Fisher, R S, Magennis, S W, Jones, A C, Höök, F, Brown, T, Kim, B H, Dahlén, A, Wilhelmsson, L M & Grøtli, M 2018, ' Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analogue ', Chemical Science, vol. 9, no. 14, pp. 3494-3502 . https://doi.org/10.1039/C7SC05448C

Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb6e1e0fa5c9f3ba57f43b759b407bc0
https://doi.org/10.1039/c7sc05448c

Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity

Autor: Rachel S. Fisher, Mattias Bood, Anders Foller Füchtbauer, Morten Grøtli, David Nobis, Marcus Wilhelmsson, Steven W. Magennis, Anita C. Jones

Publikováno v: Fisher, R S, Nobis, D, Füchtbauer, A F, Bood, M, Grøtli, M, Wilhelmsson, L M, Jones, A C & Magennis, S W 2018, ' Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity ', Physical Chemistry Chemical Physics . https://doi.org/10.1039/C8CP05496G

Fluorescent nucleobase analogues (FBAs) have many desirable features in comparison to extrinsic fluorescent labels, but they are yet to find application in ultrasensitive detection. Many of the disadvantages of FBAs arise from their short excitation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1b3b01271443e7e74aa24e0e60e91e5
https://eprints.gla.ac.uk/172289/13/172289.pdf