Virtual-'light-sheet' single-molecule localisation microscopy enables quantitative optical sectioning for super-resolution imaging.

Autor: Matthieu Palayret, Helen Armes, Srinjan Basu, Adam T Watson, Alex Herbert, David Lando, Thomas J Etheridge, Ulrike Endesfelder, Mike Heilemann, Ernest Laue, Antony M Carr, David Klenerman, Steven F Lee

Publikováno v: PLoS ONE, Vol 10, Iss 4, p e0125438 (2015)

Single-molecule super-resolution microscopy allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. Here, we demonstrate 3D sectioning with single-molecule super-resolution microscopy b

Externí odkaz: https://doaj.org/article/c2c023d829814008afe5d2b304949d1b

Within-host genomic diversity of candida albicans in healthy carriers

Autor: Christophe d'Enfert, Matthieu Palayret, Emilie Sitterlé, Natacha Sertour, Corinne Maufrais, Marie-Elisabeth Bougnoux

Publikováno v: Scientific Reports
Scientific Reports, 2019, 9 (1), pp.2563. ⟨10.1038/s41598-019-38768-4⟩
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
Scientific Reports (9), 1-12. (2019)
Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.2563. ⟨10.1038/s41598-019-38768-4⟩

Genomic variations in Candida albicans, a major fungal pathogen of humans, have been observed upon exposure of this yeast to different stresses and experimental infections, possibly contributing to subsequent adaptation to these stress conditions. Ye

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a1b54f86c4e1bf7be4541c659f7f793
https://hal.inrae.fr/hal-02622025

Single Molecule Studies of Key Processes during the Initiation of the Adaptive Immune Response

Autor: Kristina A. Ganzinger, Ricardo A. Fernandes, Peter Jönsson, Steven F. Lee, Matthieu Palayret, James McColl, Simon J. Davis, David Klenerman

T cell activation is pivotal to the initiation of the adaptive immune response which confers resistance to pathogens and plays a role in numerous diseases. Over the past decades, key protein interactions contributing to T cell activation via antigen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43de9f32333288959df2eedafeb776f6
https://ora.ox.ac.uk/objects/uuid:d7f9716b-d07e-4592-8a1c-e111e2d7cbca

Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions

Autor: Peter Jönsson, J Southcombe, Xiao-Ning Xu, Omer Dushek, Andrew James Godkin, Simon J. Davis, David Klenerman, Jamie Rossjohn, Paul Dunne, Jan Petersen, Edward J. Evans, Melissa Lever, Alexander Hudson, Lars Fugger, Ana Filipa L.O.M. Santos, Matthieu Palayret, James McColl, Gavin R. Screaton, Mathew H. Horrocks, Ricardo A. Fernandes, Tomáš Hanke, Jiandong Huo, Veronica T. Chang

Publikováno v: Jönsson, P, Southcombe, J H, Santos, A M, Huo, J, Fernandes, R A, McColl, J, Lever, M, Evans, E J, Hudson, A, Chang, V T, Hanke, T, Godkin, A, Dunne, P D, Horrocks, M H, Palayret, M, Screaton, G R, Petersen, J, Rossjohn, J, Fugger, L, Dushek, O, Xu, X-N, Davis, S J & Klenerman, D 2016, ' Remarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions ', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 20, pp. 5682-7 . https://doi.org/10.1073/pnas.1513918113

The αβ T-cell coreceptor CD4 enhances immune responses more than 1 million-fold in some assays, and yet the affinity of CD4 for its ligand, peptide-major histocompatibility class II (pMHC II) on antigen-presenting cells, is so weak that it was prev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fc1ff456905f6d9c6ee2d495cb3b008

Innovative molecular-based fluorescent nanoparticles for multicolor single particle tracking in cells

Autor: Antoine G. Godin, Mireille Blanchard-Desce, Jonathan Daniel, Laurent Cognet, Matthieu Palayret, Brahim Lounis

Publikováno v: Journal of Physics D: Applied Physics
Journal of Physics D: Applied Physics, IOP Publishing, 2016, 49, pp.84002-84002. ⟨10.1088/0022-3727/49/8/084002⟩

Based on an original molecular-based design, we present bright and photostable fluorescent organic nanoparticles (FONs) showing excellent colloidal stability in various aqueous environments. Complementary near-infrared emitting and green emitting FON

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34dc5604e9e8f25043a5629083ed5658
http://hdl.handle.net/20.500.12278/121150