Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Matthias Jöhnck"'
Publikováno v:
Journal of Separation Science. 39:663-675
The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare. This work describes a mechanistic model capturing salt a
Publikováno v:
Biotechnology progress. 34(4)
The efficiencies of mono gradient elution and dual salt-pH gradient elution for separation of six mAb charge and size variants on a preparative-scale ion exchange chromatographic resin are compared in this study. Results showed that opposite dual sal
Autor:
Joachim Kinkel, Jules Dingenen, Cedric du Fresne von Hohenesche, Michael Schulte, Klaus K. Unger, Romas Skudas, Matthias Jöhnck, Wolfgang Wewers
Publikováno v:
Preparative Chromatography, Second Edition
Autor:
Massimo Morbidelli, Guido Ströhlein, Pascal Valax, Michael Schulte, Thomas Müller-Späth, Gianni Baer, Lars Aumann, Henri Kornmann, E. Charbaut, A. Lamproye, Lydia Delegrange, Matthias Jöhnck
Publikováno v:
Biotechnology and Bioengineering. 107:974-984
A two-step chromatography process for monoclonal antibody (mAb) purification from clarified cell culture supernatant (cCCS) was developed using cation exchange Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) as a capture step. After
Autor:
Božidar Cvijetić, Agnes Franke, Michael Schulte, Nicola Forrer, Alessandro Butté, Massimo Morbidelli, Matthias Jöhnck
Publikováno v:
Journal of Chromatography A. 1217:2216-2225
The performance of functionalized materials, such as cation exchange resins, is dependent not only on the ligand type and ligand density, but also on the pore accessibility of the target molecule. In the case of large molecules such as antibodies thi
Publikováno v:
Journal of separation science. 39(4)
The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare. This work describes a mechanistic model capturing salt a
Publikováno v:
ELECTROPHORESIS. 23:774-781
The use of a poly(methylmethacrylate) capillary electrophoresis chip, provided with a high sample load capacity separation system (a 8500 nL separation channel coupled to a 500 nL sample injection channel) and a pair of on-chip conductivity detectors
Publikováno v:
Journal of Chromatography A. 916:167-174
Isotachophoretic (ITP) separation and determination of a group of 13 organic and inorganic acids, currently present in wines, on a poly(methyl methacrylate) chip provided with on-column conductivity detection was a subject of a detailed study perform
Autor:
V. Madajová, Bernd Stanislawski, Dušan Kaniansky, Matthias Jöhnck, Róbert Bodor, Marián Masár
Publikováno v:
Journal of Chromatography A. 916:155-165
The use of a poly(methylmethacrylate) chip, provided with two separation channels in the column-coupling (CC) arrangement and on-column conductivity detection sensors, to electrophoretic separations of a group of inorganic anions (chloride, nitrate,
Autor:
Friedhelm Eisenbeiß, Günther Weber, Dirk Siepe, Roland Hergenröder, Benedikt Gras, Andreas Neyer, Matthias Jöhnck
Publikováno v:
Sensors and Actuators B: Chemical. 72:249-258
This report describes the design, fabrication and use of new microanalytical devices based on polymer substrates for electrophoretic methods, especially isotachophoresis. The devices are fabricated by hot embossing and are sealed with a thin plexigla