Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Matthias Hügle"'
Autor:
Ole Behrmann, Matthias Hügle, Peter Bronsert, Bettina Herde, Julian Heni, Marina Schramm, Frank T Hufert, Gerald A Urban, Gregory Dame
Publikováno v:
PLoS ONE, Vol 14, Iss 12, p e0226571 (2019)
We present a simple to operate microfluidic chip system that allows for the extraction of miRNAs from cells with minimal hands-on time. The chip integrates thermoelectric lysis (TEL) of cells with native gel-electrophoretic elution (GEE) of released
Externí odkaz:
https://doaj.org/article/365931d93abd43e6b66f2c0f7e8ac857
Autor:
Ole Behrmann, Matthias Hügle, Franz Eckardt, Iris Bachmann, Cecilia Heller, Marina Schramm, Carrie Turner, Frank T. Hufert, Gregory Dame
Publikováno v:
Micromachines, Vol 11, Iss 6, p 595 (2020)
We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as t
Externí odkaz:
https://doaj.org/article/0fdc8a26845941e188d493e4e710f9b8
Publikováno v:
Proceedings, Vol 1, Iss 4, p 535 (2017)
We present a novel approach for the efficiency enhancement of microfluidic bacteria enrichment systems based on free-flow electrophoresis (FFE). FFE efficiency is highly dependent on the electrophoretic mobility μ of the bacteria. As μ varies stron
Externí odkaz:
https://doaj.org/article/3d5cead0f003444092ef616710b71563
Publikováno v:
The Analyst. 145:2554-2561
Nucleic acid amplification techniques such as real-time PCR are essential instruments for the identification and quantification of viruses. They are fast, very sensitive and highly specific, but often require elaborate and labor intensive sample prep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42476cb5c0d9980f736ee38ac5e9a618
https://doi.org/10.1039/c9an02333j
https://doi.org/10.1039/c9an02333j
Autor:
Johannes Wöhrle, Günter Roth, Matthias Hügle, Stefan D Krämer, Gerald Urban, Philipp A. Meyer, Christin Rath
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
Scientific Reports
Scientific Reports
In this work we show how DNA microarrays can be produced batch wise on standard microscope slides in a fast, easy, reliable and cost-efficient way. Contrary to classical microarray generation, the microarrays are generated via digital solid phase PCR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29f160626bd8373420a3e4cfe9c036f8
https://doi.org/10.1038/s41598-020-62404-1
https://doi.org/10.1038/s41598-020-62404-1
Publikováno v:
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII).
We present a lab-on-a-chip for the extraction of bacterial nucleic acids from whole human blood (WHB). Compared to classical methods, with extraction times between hours and days, the presented chip can lyse the bacteria suspended in WHB and extract
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a28fd26157aeeb012d6dbb9df3a95cdd
https://doi.org/10.1109/transducers.2019.8808273
https://doi.org/10.1109/transducers.2019.8808273
Publikováno v:
Proceedings, Vol 1, Iss 4, p 535 (2017)
We present a novel approach for the efficiency enhancement of microfluidic bacteria enrichment systems based on free-flow electrophoresis (FFE). FFE efficiency is highly dependent on the electrophoretic mobility μ of the bacteria. As μ varies stron
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87a608d4094c166a6e8edec708c166a7
https://doi.org/10.3390/proceedings1040535
https://doi.org/10.3390/proceedings1040535
Autor:
Thomas Westerhoff, Gerald Urban, Christian Heese, Verena Blättel, Dennis Elsässer, Silvia Vosseler, Michael Seidel, Matthias Hügle, Buren Scharaw, Andreas Kunze, Matthias Spoo, Reinhard Niessner, Daniel Karthe, Frank T. Hufert, Johannes Ho, Ole Behrmann, Gregory Dame, Andreas Tiehm
Publikováno v:
Environmental Earth Sciences. 75
The state-of-the-art monitoring of drinking water hygiene is based on the cultivation and enumeration of indicator bacteria. Despite its proven reliability, this approach has the disadvantages of being (a) relatively slow and (b) limited to a small n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8ea210d373f6c1dc54e57733f009653
https://doi.org/10.1007/s12665-016-6287-9
https://doi.org/10.1007/s12665-016-6287-9
Autor:
Daniel Karthe, R. Rietzel, Matthias Hügle, Frank T. Hufert, Gerald Urban, Ole Behrmann, Gregory Dame
Publikováno v:
RSC Advances. 8:26817-26817
Correction for ‘A lab-on-a-chip for preconcentration of bacteria and nucleic acid extraction’ by M. Hügle et al., RSC Adv., 2018, 8, 20124–20130.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c9306212479597927a710194fdb5aade
https://doi.org/10.1039/c8ra90058b
https://doi.org/10.1039/c8ra90058b
Publikováno v:
Biosensorsbioelectronics. 67
With a rapid and simple actuation protocol electrophoretic nucleic acid extraction is easy automatable, requires no moving parts, is easy to miniaturize and furthermore possesses a size dependent cut-off filter adjustable by the pore size of the hydr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::263c5f56536c7252b2cd1e803f2b85e9
https://pubmed.ncbi.nlm.nih.gov/24970713
https://pubmed.ncbi.nlm.nih.gov/24970713