Mapping fast DNA polymerase exchange during replication

Autor: Longfu Xu, Matthew T. J. Halma, Gijs J. L. Wuite

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)

Abstract Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like helicase. Here we present data, emp

Externí odkaz: https://doaj.org/article/3d5c500a721b415dbb08911b751ff116

Therapeutic potential of compounds targeting SARS-CoV-2 helicase

Autor: Matthew T. J. Halma, Mark J. A. Wever, Sanne Abeln, Didier Roche, Gijs J. L. Wuite

Publikováno v: Frontiers in Chemistry, Vol 10 (2022)

The economical and societal impact of COVID-19 has made the development of vaccines and drugs to combat SARS-CoV-2 infection a priority. While the SARS-CoV-2 spike protein has been widely explored as a drug target, the SARS-CoV-2 helicase (nsp13) doe

Externí odkaz: https://doaj.org/article/16550a74f2184770aad41d3ed1a5db68

Identifying Inhibitors of −1 Programmed Ribosomal Frameshifting in a Broad Spectrum of Coronaviruses

Autor: Sneha Munshi, Krishna Neupane, Sandaru M. Ileperuma, Matthew T. J. Halma, Jamie A. Kelly, Clarissa F. Halpern, Jonathan D. Dinman, Sarah Loerch, Michael T. Woodside

Publikováno v: Viruses, Vol 14, Iss 2, p 177 (2022)

Recurrent outbreaks of novel zoonotic coronavirus (CoV) diseases in recent years have highlighted the importance of developing therapeutics with broad-spectrum activity against CoVs. Because all CoVs use −1 programmed ribosomal frameshifting (−1

Externí odkaz: https://doaj.org/article/38ee90c130f449e180f7311ff75d67ca

Optical tweezers for drug discovery

Autor: Matthew T J, Halma, Jack A, Tuszynski, Gijs J L, Wuite

Publikováno v: Halma, M T J, Tuszynski, J A & Wuite, G J L 2023, ' Optical tweezers for drug discovery ', Drug Discovery Today, vol. 28, no. 1, 103443, pp. 1-12 . https://doi.org/10.1016/j.drudis.2022.103443

The time taken and the cost of producing novel therapeutic drugs presents a significant burden – a typical target-based drug discovery process involves computational screening of drug libraries, compound assays and expensive clinical trials. This r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec3b01e8f2ecca9f36af4fc44aab31f0
https://research.vu.nl/en/publications/441eb125-7fed-4462-9ab0-3984a8ab613b

Conformational Shannon Entropy of mRNA Structures from Force Spectroscopy Measurements Predicts the Efficiency of −1 Programmed Ribosomal Frameshift Stimulation

Autor: Michael T. Woodside, Dustin B. Ritchie, Matthew T. J. Halma

Publikováno v: Physical Review Letters. 126

$\ensuremath{-}1$ programmed ribosomal frameshifting ($\ensuremath{-}1\text{ }\text{ }\mathrm{PRF}$) is stimulated by structures in messenger RNA (mRNA), but the factors determining $\ensuremath{-}1\text{ }\text{ }\mathrm{PRF}$ efficiency are unclear

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::58330aecf2e6df79927e7368438ad802
https://doi.org/10.1103/physrevlett.126.038102