Zobrazeno 1 - 10
of 145
pro vyhledávání: '"Matthew P, Crump"'
Autor:
William M. Dawson, Eric J. M. Lang, Guto G. Rhys, Kathryn L. Shelley, Christopher Williams, R. Leo Brady, Matthew P. Crump, Adrian J. Mulholland, Derek N. Woolfson
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
So far most of the de novo designed proteins are for single states only. Here, the authors present the de novo design and crystal structure determination of a coiled-coil peptide that assembles into multiple, distinct conformational states under the
Externí odkaz:
https://doaj.org/article/da6fbe49cbb74470820dc44739bd5079
Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme
Autor:
Daniel W. Watkins, Jonathan M. X. Jenkins, Katie J. Grayson, Nicola Wood, Jack W. Steventon, Kristian K. Le Vay, Matthew I. Goodwin, Anna S. Mullen, Henry J. Bailey, Matthew P. Crump, Fraser MacMillan, Adrian J. Mulholland, Gus Cameron, Richard B. Sessions, Stephen Mann, J. L. Ross Anderson
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Catalytic mechanisms of enzymes are well understood, but achieving diverse reaction chemistries in re-engineered proteins can be difficult. Here the authors show a highly efficient and thermostable artificial enzyme that catalyzes a diverse array of
Externí odkaz:
https://doaj.org/article/872155373e0341fa822353dc3be89d1d
Autor:
Eveline Vriens, Dries De Ruysscher, Angus N.M. Weir, Sofie Dekimpe, Gert Steurs, Ahmed Shemy, Leentje Persoons, Ana Rita Santos, Christopher Williams, Dirk Daelemans, Matthew P. Crump, Arnout Voet, Wim De Borggraeve, Eveline Lescrinier, Joleen Masschelein
Publikováno v:
Angewandte Chemie.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57c0408c340afbe10f883f6145c8fe48
https://doi.org/10.1002/ange.202304476
https://doi.org/10.1002/ange.202304476
Publikováno v:
Bowen, J I, Wang, L, Crump, M P & Willis, C L 2022, ' Synthetic and Biosynthetic Methods for Selective Cyclisations of 4,5-Epoxy Alcohols to Tetrahydropyrans ', Organic and Biomolecular Chemistry, vol. 20, no. 6, 20, pp. 1150-1175 . https://doi.org/10.1039/D1OB01905H
Tetrahydropyrans (THPs) are common structural motifs found in natural products and synthetic therapeutic molecules. In Nature these 6-membered oxygen heterocycles are often assembled via intramolecular reactions involving either oxy-Michael additions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1efc70b88d4000763ae515d7bbe3e3fc
https://doi.org/10.1039/d1ob01905h
https://doi.org/10.1039/d1ob01905h
Autor:
Andrew J. Devine, Alice E. Parnell, Catherine R. Back, Nicholas R. Lees, Samuel T. Johns, Ainul Z. Zulkepli, Rob Barringer, Katja Zorn, James E. M. Stach, Matthew P. Crump, Martin A. Hayes, Marc W. van der Kamp, Paul R. Race, Christine L. Willis
Publikováno v:
Angewandte Chemie. 135
Abyssomicin C and its atropisomer are potent inhibitors of bacterial folate metabolism. They possess complex polycyclic structures, and their biosynthesis has been shown to involve several unusual enzymatic transformations. Using a combination of syn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::192ed9536efc507314b7a184d358c2a3
https://doi.org/10.1002/ange.202213053
https://doi.org/10.1002/ange.202213053
Autor:
Gemma LM Fisher, César L Pastrana, Victoria A Higman, Alan Koh, James A Taylor, Annika Butterer, Timothy Craggs, Frank Sobott, Heath Murray, Matthew P Crump, Fernando Moreno-Herrero, Mark S Dillingham
Publikováno v:
eLife, Vol 6 (2017)
The ParB protein forms DNA bridging interactions around parS to condense DNA and earmark the bacterial chromosome for segregation. The molecular mechanism underlying the formation of these ParB networks is unclear. We show here that while the central
Externí odkaz:
https://doaj.org/article/f8d8ca5b88b446fca82c3cb8f5b803c0
Autor:
Ashley J. Winter, Matthew T. Rowe, Angus N. M. Weir, Nahida Akter, Sbusisiwe Z. Mbatha, Paul D. Walker, Christopher Williams, Zhongshu Song, Paul R. Race, Christine L. Willis, Matthew P. Crump
Publikováno v:
Winter, A J, Rowe, M T, Weir, A N M, Akter, N, Mbatha, S Z, Walker, P D, Williams, C, Song, Z, Race, P R, Willis, C L & Crump, M P 2022, ' Programmed Iteration Controls the Assembly of the Nonanoic Acid Side Chain of the Antibiotic Mupirocin ', Angewandte Chemie-International Edition, vol. 61, no. 50, e202212393 . https://doi.org/10.1002/anie.202212393
Mupirocin is a clinically important antibiotic produced by Pseudomonas fluorescens NCIMB 10586 that is assembled by a complex trans-AT polyketide synthase. The polyketide fragment, monic acid, is esterified by a 9-hydroxynonanoic acid (9HN) side chai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8f3287f7bbdf8f76bb39e10e426174f
https://pubmed.ncbi.nlm.nih.gov/36227272
https://pubmed.ncbi.nlm.nih.gov/36227272
Publikováno v:
Organic & Biomolecular Chemistry. 19:6210-6215
The ambruticins are a family of polyketide natural products which exhibit potent antifungal activity. Gene knockout experiments are in accord with the proposal that the tetrahydropyran ring of the ambruticins is formed via the AmbJ catalysed epoxidat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e06baf9ce57a2df46c11dfe8cac39ab2
https://doi.org/10.1039/d1ob00883h
https://doi.org/10.1039/d1ob00883h
In type II polyketide synthases (PKSs), which typically biosynthesize several antibiotic and antitumor compounds, the substrate is a growing polyketide chain, shuttled between individual PKS enzymes whilst covalently tethered to an acyl carrier prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1f82d6f0a6c4c8432ce781dc0eb303c
https://doi.org/10.26434/chemrxiv-2021-wdlmj-v2
https://doi.org/10.26434/chemrxiv-2021-wdlmj-v2
Autor:
Zhongshu Song, Paul R. Race, Thomas J. Simpson, Christine L. Willis, Luoyi Wang, Matthew P. Crump, James Spencer
Publikováno v:
Chemical Science
Wang, L, Song, Z, Race, P R, Spencer, J, Simpson, T J, Crump, M P & Willis, C L 2020, ' Mixing and Matching Genes of Marine and Terrestrial Origin in the Biosynthesis of the Mupirocin Antibiotics ', Chemical Science, vol. 11, pp. 5221-5226 . https://doi.org/10.1039/C9SC06192D
Wang, L, Song, Z, Race, P R, Spencer, J, Simpson, T J, Crump, M P & Willis, C L 2020, ' Mixing and Matching Genes of Marine and Terrestrial Origin in the Biosynthesis of the Mupirocin Antibiotics ', Chemical Science, vol. 11, pp. 5221-5226 . https://doi.org/10.1039/C9SC06192D
With growing understanding of the underlying pathways of polyketide biosynthesis, along with the continual expansion of the synthetic biology toolkit, it is becoming possible to rationally engineer and fine-tune the polyketide biosynthetic machinery
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4507f2147d0cfad2182b049321a14299
http://europepmc.org/articles/PMC8159325
http://europepmc.org/articles/PMC8159325