Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing

Autor: Ramya Krishnan, Christine Guthrie, Nils G. Walter, Matthew L. Kahlscheuer, John Abelson, Mario Blanco

Publikováno v: Nature structural & molecular biology
Guthrie, Christine; Krishnan, R; Blanco, MR; Kahlscheuer, ML; Abelson, J; & Walter, NG. (2013). Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/538281rf

The spliceosome is a dynamic ribonucleoprotein (RNP) machine that catalyzes the removal of introns during the two transesterification steps of eukaryotic pre-mRNA splicing. Here we used single-molecule fluorescence resonance energy transfer to monito

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::813e7be4b40d06f00b8737a24ec528d1
https://doi.org/10.1038/nsmb.2704

Meeting report: SMART timing--principles of single molecule techniques course at the University of Michigan 2014

Autor: Rebecca M, Bartke, Elizabeth L, Cameron, Ajitha S, Cristie-David, Thomas C, Custer, Maxwell S, Denies, May, Daher, Soma, Dhakal, Soumi, Ghosh, Laurie A, Heinicke, J Damon, Hoff, Qian, Hou, Matthew L, Kahlscheuer, Joshua, Karslake, Adam G, Krieger, Jieming, Li, Xiang, Li, Paul E, Lund, Nguyen N, Vo, Jun, Park, Sethuramasundaram, Pitchiaya, Victoria, Rai, David J, Smith, Krishna C, Suddala, Jiarui, Wang, Julia R, Widom, Nils G, Walter

Publikováno v: Biopolymers. 103(5)

Four days after the announcement of the 2014 Nobel Prize in Chemistry for “the development of super-resolved fluorescence microscopy” based on single molecule detection, the Single Molecule Analysis in Real-Time (SMART) Center at the University o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e63c518fd139db7bf56bad3ccc540988
https://pubmed.ncbi.nlm.nih.gov/25546606

Single Molecule FRET Characterization of Pre-mRNA Splicing: Substrate Dynamics during Recognition and Catalysis

Autor: Mario Blanco, Matthew L. Kahlscheuer, Nils G. Walter, Christine Guthrie, John Abelson, Ramya Krishnan

Publikováno v: ResearcherID

The spliceosome is a complex ribonucleoprotein (RNP) that catalyzes the removal of intervening sequences (introns) from coding regions (exons) in eukaryotic pre-mRNAs. The ordered assembly of the spliceosome makes it a compositionally dynamic system

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfd0275e3681c5c2821b39fe34605a2b
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000321561201713&KeyUID=WOS:000321561201713