Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Matthew H. Zinselmeier"'
Publikováno v:
Frontiers in Genome Editing, Vol 2 (2021)
The production of transgenic or gene edited plants requires considerable time and effort. It is of value to know at the onset of a project whether the transgenes or gene editing reagents are functioning as predicted. To test molecular reagents transi
Externí odkaz:
https://doaj.org/article/835de2c19a3f4bab8ef807ef22c22c1a
Publikováno v:
Nucleic Acids Research.
CRISPR/Cas-based transcriptional activators have been developed to induce gene expression in eukaryotic and prokaryotic organisms. The main advantages of CRISPR/Cas-based systems is that they can achieve high levels of transcriptional activation and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c238918190be81027c6ef3d90ba1441e
https://doi.org/10.1093/nar/gkad458
https://doi.org/10.1093/nar/gkad458
Autor:
Matthew H Zinselmeier, J. Armando Casas-Mollano, Adam Sychla, Stephen C Heinsch, Daniel F Voytas, Michael J Smanski
Understanding how the expression of genes impacts plant development and physiology is important for rationally engineering crop improvements. Programmable Transcription Activators (PTAs), including CRISPRa activators, have traditionally relied on a l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7aa7c2e3251a429fb57fd17f83d50c7b
https://doi.org/10.1101/2022.06.10.495638
https://doi.org/10.1101/2022.06.10.495638
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2464
Recent advances in DNA synthesis and assembly allow for genetic constructs to be designed and constructed in high throughput. Characterizing large numbers of variant genetic designs is not feasible with low-throughput and time-consuming plant transfo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::83f39c48a324aa95fbe569d503155c74
https://pubmed.ncbi.nlm.nih.gov/35258836
https://pubmed.ncbi.nlm.nih.gov/35258836
Publikováno v:
CRISPR J
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in differen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f6a37223d76d9d22baf6e0f134a1c82
https://doi.org/10.1089/crispr.2020.0064
https://doi.org/10.1089/crispr.2020.0064
Publikováno v:
Methods in Molecular Biology ISBN: 9781071621639
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::84c4a886d2d9f7bedf49f0b11bbb217d
https://doi.org/10.1007/978-1-0716-2164-6_16
https://doi.org/10.1007/978-1-0716-2164-6_16