Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Mats G. L. Gustafsson"'
Publikováno v:
Journal of microscopy. 273(1)
Structured-illumination microscopy allows widefield fluorescence imaging with resolution beyond the classical diffraction limit. Its linear form extends resolution by a factor of two, and its nonlinear form by an in-principle infinite factor, the eff
Autor:
Sara Abrahamsson, Jan Wisniewski, Xavier Darzacq, Dugast Darzacq Claire, Cornelia I. Bargmann, Florian Mueller, Carl Wu, Pierre Soule, Bassam Hajj, Gaku Mizuguchi, Mats G. L. Gustafsson, Maxime Dahan, Jiji Chen, Alexander Y. Katsov, David A. Agard, Sjoerd Stallinga
Publikováno v:
Nature Methods. 10:60-63
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal
Autor:
M. Cristina Cardoso, Brian Burke, Heinrich Leonhardt, Sebastian Haase, Lin Shao, Peter M. Carlton, Mats G. L. Gustafsson, John W. Sedat, David A. Agard, Peter Kner, Lukman Winoto, Lothar Schermelleh
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0230c2d927de00f3764e93150dcdf9ed
https://ora.ox.ac.uk/objects/uuid:77841673-47eb-45d4-9ba9-b95c87e00db7
https://ora.ox.ac.uk/objects/uuid:77841673-47eb-45d4-9ba9-b95c87e00db7
Publikováno v:
Journal of Microscopy
In an interferometer-based fluorescence microscope, a beam splitter is often used to combine two emission wavefronts interferometrically. There are two perpendicular paths along which the interference fringes can propagate and normally only one is us
Publikováno v:
Nature methods
Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 10
Autor:
C. J. Rachel Wang, John W. Sedat, David A. Agard, Lin Shao, W. Zacheus Cande, Peter M. Carlton, Inna N. Golubovskaya, Mats G. L. Gustafsson
Publikováno v:
Biophysical Journal. 94(12):4957-4970
Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied
Publikováno v:
Proceedings of the National Academy of Sciences. 98:3790-3795
Light microscopy of thick biological samples, such as tissues, is often limited by aberrations caused by refractive index variations within the sample itself. This problem is particularly severe for live imaging, a field of great current excitement d
Autor:
Mats G. L. Gustafsson
Publikováno v:
Journal of Microscopy. 198:82-87
Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide-field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, w
Publikováno v:
Nature Photonics. 3:362-364
Super-resolution light microscopy methods either localize single molecular labels or treat the sample as a continuous object. The fundamental requirements for super-resolution in the continuum regime are spatially non-uniform illumination and a nonli
Autor:
Mats G. L. Gustafsson
Publikováno v:
Nature Methods. 5:385-387
Microscopic resolution far beyond the diffraction limit is possible by localizing single molecules individually. This approach has now been demonstrated on living cells.