New Disulphide Bond in Cystatin-Based Protein Scaffold Prevents Domain-Swap-Mediated Oligomerization and Stabilizes the Functionally Active Form

Autor: Matja Zalar, Alexander P. Golovanov

Publikováno v: ACS Omega, Vol 4, Iss 19, Pp 18248-18256 (2019)

Externí odkaz: https://doaj.org/article/6f04a79e79ed414697982ecf21b0e09a

Insights into the Stabilization of Interferon Alpha by Two Surfactants Revealed by STD-NMR Spectroscopy

Autor: Hristo L. Svilenov, Katharina T. Kopp, Alexander P. Golovanov, Gerhard Winter, Matja Zalar

Publikováno v: JOURNAL OF PHARMACEUTICAL SCIENCES

Surfactants are commonly used in biopharmaceutical formulations to stabilize proteins against aggregation. However, the choice of a suitable surfactant for a particular protein is decided mostly empirically, and their mechanism of action on molecular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73c38d51496a10eb98b4cef70bd6a127
https://doi.org/10.1016/j.xphs.2022.10.013

Nonspecific Binding of Adenosine Tripolyphosphate and Tripolyphosphate Modulates the Phase Behavior of Lysozyme

Autor: Matja Zalar, Jordan Bye, Robin Curtis

Publikováno v: Zalar, M, Bye, J & Curtis, R 2023, ' Nonspecific Binding of Adenosine Tripolyphosphate and Tripolyphosphate Modulates the Phase Behavior of Lysozyme ', Journal of the American Chemical Society, vol. 145, no. 2, pp. 929-943 . https://doi.org/10.1021/jacs.2c09615

Adenosine tripolyphosphate (ATP) is a small polyvalent anion that has recently been shown to interact with proteins and have a major impact on assembly processes involved in biomolecular condensate formation and protein aggregation. However, the natu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::928bf690b505f827d384e974995345fa
https://research.manchester.ac.uk/en/publications/e677a526-8806-47b5-8434-f39a3dec747d

Controlled release and characterisation of photocaged molecules using in situ LED illumination in solution NMR spectroscopy

Autor: Jack E. Bramham, Matja Zalar, Alexander P. Golovanov

Publikováno v: Bramham, J E, Zalar, M & Golovanov, A P 2022, ' Controlled release and characterisation of photocaged molecules using in situ LED illumination in solution NMR spectroscopy ', Chemical Communications, vol. 58, no. 85, pp. 11973-11976 . https://doi.org/10.1039/D2CC04731D

Photocaging is an attractive strategy to control molecular behaviour, for example, in chemical synthesis, interaction studies or photodynamic therapies. Here, we demonstrate that in situ illumination by the LED NMRtorch approach enables effective and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ed70918ba91f415fdd4ad9e02510e9
https://uom.elsevierpure.com/en/publications/22a1060b-e7c4-48e7-a9b4-bea735267e7c

Development of a Fast Screening Method for Selecting Excipients in Formulations using MD simulations, NMR and MicroScale Thermophoresis

Autor: Werner Streicher, Christin Pohl, Alexander P. Golovanov, Pernille Harris, Matja Zalar, Sowmya Indrakumar, Nuska Tschammer, Allan Nørgaard, Günther H.J. Peters

Publikováno v: Indrakumar, S, Zalar, M, Tschammer, N, Pohl, C A, Nørgaard, A, Streicher, W, Harris, P, Golovanov, A P & Peters, G H J 2021, ' Development of a Fast Screening Method for Selecting Excipients in Formulations using MD simulations, NMR and MicroScale Thermophoresis ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 158, pp. 11-20 . https://doi.org/10.1016/j.ejpb.2020.10.015

Development of peptide therapeutics generally involves screening of excipients that inhibit peptide-peptide interactions, hence aggregation, and improve peptide stability. We used the therapeutic peptide plectasin to develop a fast screening method t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a53ed78bec97c51dee0259e918874e4
https://orbit.dtu.dk/en/publications/4305e7c3-4649-4809-9959-fe8436ce07c3