Accurate Evaluation of Hepatocyte Metabolisms on a Noble Oxygen-Permeable Material With Low Sorption Characteristics

Autor: Masaki Nishikawa, Hiroyasu Ito, Fumiya Tokito, Keita Hirono, Kousuke Inamura, Benedikt Scheidecker, Mathieu Danoy, Takumi Kawanishi, Hirohsi Arakawa, Yukio Kato, Katsuhiro Esashika, Hiroshi Miyasako, Yasuyuki Sakai

Publikováno v: Frontiers in Toxicology, Vol 4 (2022)

In the pharmaceutical industry, primary cultured hepatocytes is a standard tool used to assess hepatic metabolisms and toxicity in vitro. Drawbacks, however, include their functional deterioration upon isolation, mostly due to the lack of a physiolog

Externí odkaz: https://doaj.org/article/6b9bd2ba86104b5498c6de2c0db815e1

Induction of in vitro Metabolic Zonation in Primary Hepatocytes Requires Both Near-Physiological Oxygen Concentration and Flux

Autor: Benedikt Scheidecker, Marie Shinohara, Masahiro Sugimoto, Mathieu Danoy, Masaki Nishikawa, Yasuyuki Sakai

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

Pre-clinical drug screening is an important step in assessing the metabolic effects and hepatic toxicity of new pharmaceutical compounds. However, due to the complexity of the liver microarchitecture, simplified in vitro models do not adequately refl

Externí odkaz: https://doaj.org/article/6274cd11b23345fdafc581da779630fb

Transcriptomic and proteomic studies suggest the establishment of advanced zonation‐like profiles in human‐induced pluripotent stem cell‐derived liver sinusoidal endothelial cells and carboxypeptidase M‐positive liver progenitor cells cocultured in a fluidic microenvironment

Autor: Mathieu Danoy, Stéphane Poulain, Rachid Jellali, Benedikt Scheidecker, Yannick Tauran, Marjorie Leduc, Johanna Bruce, Soo Hyeon Kim, Taketomo Kido, Atsushi Miyajima, Yasuyuki Sakai, Eric Leclerc

Publikováno v: Hepatology Research.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d0b4d54ae0249607a355e4d3c3a3f676
https://doi.org/10.1111/hepr.13893

Characterization of the proteome and metabolome of human liver sinusoidal endothelial-like cells derived from induced pluripotent stem cells

Autor: Benedikt Scheidecker, Marjorie Leduc, Françoise Gilard, Rachid Jellali, Eric Leclerc, Bertrand Gakière, Atsushi Miyajima, Mathieu Danoy, Taketomo Kido, Yasuyuki Sakai, Fabrice Soncin, Johanna Bruce, Yannick Tauran

Publikováno v: Differentiation
Differentiation, Elsevier, 2021, 120, pp.28-35. ⟨10.1016/j.diff.2021.06.001⟩
Differentiation, 2021, 120, pp.1-26. ⟨10.1016/j.diff.2021.06.001⟩

International audience; The liver is a complex organ composed of several cell types organized hierarchically. Among these, liver sinusoidal endothelial cells (LSECs) are specialized vascular cells known to interact with hepatocytes and hepatic stella

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e74998e41866c9c53fae07a57defad0
https://doi.org/10.1016/j.diff.2021.06.001

Organization of liver organoids using Raschig ring-like micro-scaffolds and triple co-culture: Toward modular assembly-based scalable liver tissue engineering

Autor: Stephanie Sutoko, Kevin Montagne, Yuan Pang, Toshiki Niino, Yohei Horimoto, Yasuyuki Sakai, Ikki Horiguchi, Marie Shinohara, Zitong Wang, Mathieu Danoy

Publikováno v: Medical Engineering & Physics. 76:69-78

In order to address the remaining issues of fragile structure and insufficient mass transfer faced in modular assembly-based liver tissue engineering, a Raschig ring-like hollowed micro-scaffold was proposed and fabricated using poly-e-caprolactone w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::174c55323190bc0ec42176d220a603fa
https://doi.org/10.1016/j.medengphy.2019.10.010