Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro

Autor: Matheus Silva e Bastos, Luciana Ângelo de Souza, Thiago Souza Onofre, Abelardo Silva Júnior, Márcia Rogéria de Almeida, Gustavo Costa Bressan, Juliana Lopes Rangel Fietto

Publikováno v: Memorias do Instituto Oswaldo Cruz, Vol 112, Iss 2, Pp 155-159

BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS

Externí odkaz: https://doaj.org/article/96638461f12f4193b590d5a431e34301

The expression of NTPDase1 and -2 of Leishmania infantum chagasi in bacterial and mammalian cells: Comparative expression, refolding and nucleotidase characterization

Autor: Abelardo Silva-Júnior, Alain Tremblay, I.L.A. Rabelo, L.P. Barreto, Juliana Lopes Rangel Fietto, Gustavo Costa Bressan, Joice de Melo Agripino, Matheus Silva e Bastos, Jean Sévigny, Márcia Rogéria de Almeida, Julie Pelletier, Joanna Lecka

Publikováno v: LOCUS Repositório Institucional da UFV
Universidade Federal de Viçosa (UFV)
instacron:UFV

Visceral Leishmaniasis (VL) represents an important global health problem in several warm countries around the world. The main targets in this study are the two nucleoside triphosphate diphosphohy- drolases (NTPDases) from Leishmania infantum chagasi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f54c495bdc83ec18d1d3f5ed9313204
https://pubmed.ncbi.nlm.nih.gov/27856402

Leishmania infantum ecto-nucleoside triphosphate diphosphohydrolase-2 is an apyrase involved in macrophage infection and expressed in infected dogs

Autor: Aimara da Costa Pinheiro, Gabriel Andrés Tafur Gomez, Rodrigo Saar Gomes, Munira Muhammad Abdel Baqui, Gustavo Costa Bressan, Abelardo Silva-Júnior, Lucas Borges-Pereira, Anna Cláudia Alves de Souza, Matheus Silva e Bastos, Ronny Francisco de Souza, Luís Carlos Crocco Afonso, Márcia Rogéria de Almeida, Rafaela de Cássia Firmino, Christiane Mariotini-Moura, Claudia Miranda de Oliveira, Tiago D. Serafim, Talles Eduardo Ferreira Maciel, Felipe Freitas de Castro, Raphael de Souza Vasconcellos, Juliana Lopes Rangel Fietto

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS Neglected Tropical Diseases
LOCUS Repositório Institucional da UFV
Universidade Federal de Viçosa (UFV)
instacron:UFV
PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3309 (2014)

Background Visceral leishmaniasis is an important tropical disease, and Leishmania infantum chagasi (synonym of Leishmania infantum) is the main pathogenic agent of visceral leishmaniasis in the New World. Recently, ecto-nucleoside triphosphate dipho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2e2d9747e4caae38d31386ec04edd32

Influence of ecto-nucleoside triphosphate diphosphohydrolase activity on Trypanosoma cruzi infectivity and virulence

Autor: Sergio Verjovski-Almeida, Márcia Rogéria de Almeida, Matheus Silva e Bastos, Ricardo DeMarco, Maria Terezinha Bahia, Marcela A. S. Pôssa, Paulo Marcos da Matta Guedes, Juliana Lopes Rangel Fietto, Ramon de Freitas Santos

Publikováno v: Repositório Institucional da UFOP
Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, Vol 3, Iss 3, p e387 (2009)

Background The protozoan Trypanosoma cruzi is the causative agent of Chagas disease. There are no vaccines or effective treatment, especially in the chronic phase when most patients are diagnosed. There is a clear necessity to develop new drugs and s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f3bfa9821798d14ffd33f6499e24c5e
http://www.repositorio.ufop.br/handle/123456789/4609