Zobrazeno 1 - 10
of 55
pro vyhledávání: '"Masuyo Nakai"'
Publikováno v:
Journal of Microbiological Methods. 77:139-144
Mycobacterial cell wall consists of rigid cell wall skeleton (CWS), a mycoloyl arabinogalactan peptidiglycan complex, in which mycoloyl structure varies by the mycobacterial species diversely, whereas the arabinogalactan peptidoglycan structure is co
Publikováno v:
Micron. 29:123-138
The life-cycle of human immunodeficiency virus type 1 (HIV-1) has been studied using several techniques including immunoelectron microscopy and cryomicroscopy. The HIV-1 particle consists of an envelope, a core and the region between the core and the
Publikováno v:
AIDS Research and Human Retroviruses. 12:793-800
To clarify the physiological function of two zinc finger motifs in the nucleocapsid (NC) domain of the Gag protein of human immunodeficiency virus type 1 (HIV-1), we changed cysteine to serine in either of the two motifs or both by site-directed muta
Publikováno v:
Journal of Virological Methods. 58:199-204
The sensitivities of two reverse transcriptase (RT) assays, an enzyme-linked oligonucleotide sorbent assay (ELOSA)-RT assay and a non-radioisotopic (non-RI) RT assay were compared. For measuring recombinant HIV-1 RT, the ELOSA-RT assay was 8 times le
Autor:
Chikara Aizawa, Satoshi Ō mura, Toshiyuki Goto, Setsuko Hinata, Yuko Morikawa, Hiroshi Tomoda, Haruo Tanaka, Masuyo Nakai
Publikováno v:
Journal of Biological Chemistry. 271:2868-2873
Myristoylation of human immunodeficiency virus (HIV) Gag protein is essential for virus particle budding. Two reactions are involved; activation of free myristate to myristoyl-CoA and transfer of the myristoyl residue to the Gag N-terminal glycine. W
Autor:
Susumu Okubo, Toru Otake, Noboru Ueba, Haruyo Mori, Kouichi Sano, Kojiro Yasunaga, Motoko Morimoto, Masuyo Nakai, Takashi Nakano, Takuya Kawahata
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 70:347-353
We have reported on the investigation of 54 HIV-1 patients concerning the isolation and clinical marker in the preceding paper. We have attempted the analysis of the V3 and RT genes. HIV-1 from a patient who had rapidly taken a turn for the worse had
Autor:
Haruyo Mori, Masuyo Nakai, Susumu Okubo, Noboru Ueba, Yasuto Okubo, Takuya Kawahata, Nobuo Nagao, Motoko Morimoto, Toru Otake, Kouichi Sano, Kojiro Yasunaga, Takashi Nakano
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 70:338-346
We followed 54 HIV-1 carriers (44 asymptomatic carriers and 10 AIDS patients) by virus isolation and immunological examination and evaluated their usefulness for prognostication of the onset of symptoms. From 37 carriers (27 asymptomatic carriers and
Autor:
Masuyo Nakai, Fumitomo Odawara, Hideo Misaki, Yukiko Saitoh, Katsuhide Hayashi, Takashi Nakano, Toru Otake, Tsumukata Nakamura, Kouichi Sano
Publikováno v:
Journal of the Japanese Association for Infectious Diseases. 68:923-931
We developed an improved, highly sensitive non-radioisotopic (non-RI) reverse transcriptase (RT) assay (RTA). While the original non-RI method previously reported made use of primer immobilization , our improved method was based on a primer-template
Publikováno v:
Microscopy Research and Technique. 25:335-340
The cores of human and simian immunodeficiency viruses (HIV and SIV) were observed by negative staining after isolation of the core with Nonidet P40 and glutaraldehyde. Four kinds of cores were found: asymmetric and symmetric sectoral shapes, a bar s
Autor:
Meiko Kawamura, M. Suleman, Jun Katahira, Toshiyuki Goto, Keizō Tomonaga, Tomoyuki Miura, Masuyo Nakai, Masashi Fukasawa, M. Isahakia, M. A. Hassan, Hirofumi Akari, Masanori Hayami
Publikováno v:
Archives of Virology. 129:77-92
Forty-eight of 236 sera from seven species of African non-human primates in Kenya, including those of white-crowned mangabey monkeys (Cercocebus torquatus lunulatus) had antibodies to simian immunodeficiency viruses (SIVs). Isolates of simian lentivi