Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.

Autor: Massengill CI; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Bayless-Edwards L; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Ceballos CC; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Cebul ER; Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA., Cahill J; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Bharadwaj A; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Wilson E; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Qin M; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Whorton MR; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Baconguis I; Vollum Institute, Oregon Health & Science University, Portland, OR, USA., Ye B; Life Sciences Institute and Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, USA., Mao T; Vollum Institute, Oregon Health & Science University, Portland, OR, USA. mao@ohsu.edu., Zhong H; Vollum Institute, Oregon Health & Science University, Portland, OR, USA. zhong@ohsu.edu.

Publikováno v: Nature methods [Nat Methods] 2022 Nov; Vol. 19 (11), pp. 1461-1471. Date of Electronic Publication: 2022 Oct 27.

High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion.

Autor: Zhong H; Vollum Institute, Oregon Health & Science University, Portland, United States., Ceballos CC; Vollum Institute, Oregon Health & Science University, Portland, United States., Massengill CI; Vollum Institute, Oregon Health & Science University, Portland, United States., Muniak MA; Vollum Institute, Oregon Health & Science University, Portland, United States., Ma L; Vollum Institute, Oregon Health & Science University, Portland, United States., Qin M; Vollum Institute, Oregon Health & Science University, Portland, United States., Petrie SK; Department of Neurology, Oregon Health & Science University, Portland, United States., Mao T; Vollum Institute, Oregon Health & Science University, Portland, United States.

Publikováno v: ELife [Elife] 2021 Jun 08; Vol. 10. Date of Electronic Publication: 2021 Jun 08.

Cell-Specific Single Viral Vector CRISPR/Cas9 Editing and Genetically Encoded Tool Delivery in the Central and Peripheral Nervous Systems.

Autor: Moffa, Jamie C., Bland, India N., Tooley, Jessica R., Kalyanaraman, Vani, Heitmeier, Monique, Creed, Meaghan C., Copits, Bryan A.

Publikováno v: eNeuro; Jul2024, Vol. 11 Issue 7, p1-23, 23p

A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.

Autor: Kaczmarczyk, Andreas, van Vliet, Simon, Jakob, Roman Peter, Teixeira, Raphael Dias, Scheidat, Inga, Reinders, Alberto, Klotz, Alexander, Maier, Timm, Jenal, Urs

Publikováno v: Nature Communications; 5/9/2024, Vol. 15 Issue 1, p1-18, 18p

qTAG: an adaptable plasmid scaffold for CRISPR-based endogenous tagging.

Autor: Philip R; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 3E1, Canada., Sharma A; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada., Matellan L; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada., Erpf AC; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada., Hsu WH; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada., Tkach JM; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada., Wyatt HDM; Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8, Canada., Pelletier L; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada. pelletier@lunenfeld.ca.; Department of Molecular Genetics, University of Toronto, Toronto, ON, M5S 3E1, Canada. pelletier@lunenfeld.ca.

Publikováno v: The EMBO journal [EMBO J] 2024 Dec 12. Date of Electronic Publication: 2024 Dec 12.