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pro vyhledávání: '"Masoud R. S. Shapouri"'
Publikováno v:
Avian Pathology. 26:419-425
An E. coli-expressed fusion protein was used to study the immunogenicity of avian reovirus sigma3 protein in chickens. The protein induced the production of specific antibodies detectable by immunofluorescence, ELISA and immunoblot. The antibody titr
Autor:
Masoud R. S. Shapouri, Jean Bergeron, Micheline Letarte, Max Arella, Marième Kane, Amer Silim
Publikováno v:
Journal of General Virology. 76:1515-1520
The S1 genome segment of avian reovirus strain S1133 was cloned and completely sequenced. The sequence comprised 1636 bp with three distinct open reading frames (ORFs), suggesting the gene was polycistronic in nature. The three ORFs from 5' to 3' wer
Publikováno v:
The Journal of general virology. 77
It has been suggested that avian reovirus sigma 3 protein is analogous to sigma 1 trimer, the mammalian reovirus attachment protein. We have investigated the multimeric nature and cell binding ability of sigma 3 protein. The data presented here demon
Publikováno v:
Avian pathology : journal of the W.V.P.A. 25(1)
Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization of six MAbs in Western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the MAbs were specific to the ou