Výsledky vyhledávání - "Mason R. Mackey"

Elemental mapping of labelled biological specimens at intermediate energy loss in an energy‐filtered TEM acquired using a direct detection device

Autor: Mark H. Ellisman, Steven T. Peltier, Stephen R. Adams, Xuong Nguyen-Huu, Junru Hu, Ranjan Ramachandra, Mason R. Mackey

Publikováno v: Journal of Microscopy
Journal of microscopy, vol 283, iss 2

The technique of colour EM that was recently developed enabled localisation of specific macromolecules/proteins of interest by the targeted deposition of diaminobenzidine (DAB) conjugated to lanthanide chelates. By acquiring lanthanide elemental maps

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::727236fe7819ee8cf4be6346cc1047b0
https://doi.org/10.1111/jmi.13014

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Genetic Probe for Visualizing Glutamatergic Synapses and Vesicles by 3D Electron Microscopy

Autor: Carolina Li, Matthew Madany, Matthias G. Haberl, Vivien Zell, Stephen R. Adams, Daniela Boassa, Junru Hu, Thomas Steinkellner, Thomas S. Hnasko, Mason R. Mackey, Mark H. Ellisman, Ranjan Ramachandra

Publikováno v: ACS Chem Neurosci

[Image: see text] Communication between neurons relies on the release of diverse neurotransmitters, which represent a key-defining feature of a neuron’s chemical and functional identity. Neurotransmitters are packaged into vesicles by specific vesi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6b77dbfff2dcfa9f65a672ffc076d6d
https://pubmed.ncbi.nlm.nih.gov/33522227

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A genetic probe for visualizing glutamatergic synapses and vesicles by 3D electron microscopy

Autor: Thomas Steinkellner, Daniela Boassa, Mark H. Ellisman, Stephen Adams, Matthew Madany, Thomas S. Hnasko, Vivien Zell, Mason R. Mackey, Matthias G. Haberl, Ranjan Ramachandra, Junru Hu, Carolina Li

Communication between neurons relies on the release of diverse neurotransmitters, which represent a key-defining feature of a neuron’s chemical and functional identity. Neurotransmitters are packaged into vesicles by specific vesicular transporters

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::694893f1ce2a072d244626029fc3481c
https://doi.org/10.1101/2020.07.31.230995

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Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy

Autor: John T. Ngo, Stephen R. Adams, Mark H. Ellisman, Sakina F Palida Lemieux, Mason R. Mackey, Varda Lev-Ram, Daniela Boassa, Qing Xiong, Ranjan Ramachandra, Junru Hu, Sebastien Phan, Ryan Emily Peace

Publikováno v: Cell chemical biology, vol 26, iss 10
Cell Chem Biol

A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 domain of Arab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::740cef92bd07fcb272398aed008522b2
https://escholarship.org/uc/item/34q0x5jb

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Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species

Autor: Eric A. Bushong, Roger Y. Tsien, Sakina F Palida Lemieux, Stephen R. Adams, Mark H. Ellisman, Paul Steinbach, Mason R. Mackey, Ranjan Ramachandra, Margaret T. Butko, Ben N G Giepmans

Publikováno v: Cell chemical biology, vol 23, iss 11
Cell Chemical Biology, 23(11), 1417-1427. CELL PRESS

Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::902dc68568afd8faad46a672ef934c63
https://doi.org/10.1016/j.chembiol.2016.10.006

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Subgroup characteristics of marine methane-oxidizing ANME-2 archaea and their syntrophic partners revealed by integrated multimodal analytical microscopy

Autor: Mason R. Mackey, Thomas J. Deerinck, Andrea Thor, Grayson L. Chadwick, Mark H. Ellisman, Shawn E. McGlynn, Ariel O'Neill, Victoria J. Orphan

Phylogenetically diverse environmental ANME archaea and sulfate-reducing bacteria cooperatively catalyze the anaerobic oxidation of methane oxidation (AOM) in multicelled consortia within methane seep environments. To better understand these cells an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e234f54787b069128b3d0ade8a5efc3d
https://resolver.caltech.edu/CaltechAUTHORS:20180411-080037971

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Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors

Autor: Nobuko Fujikawa, Mason R. Mackey, Tom Case, Eric A. Bushong, Keunyoung Kim, Matthias G. Haberl, Thomas J. Deerinck, Tsvi Katchalski, Ranjan Ramachandra, Guillaume A. Castillon, Steven T. Peltier, Mark H. Ellisman, Albert R. Lawrence

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific reports, vol 8, iss 1
Scientific Reports

Biological samples are frequently stained with heavy metals in preparation for examining the macro, micro and ultra-structure using X-ray microtomography and electron microscopy. A single X-ray microtomography scan reveals detailed 3D structure based

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94cfaaad307c7ce7d7ddee9c219c01c0
https://doi.org/10.1038/s41598-018-25099-z

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Sub-mitochondrial localization of genetic-tagged MIB interacting partners: Mic19, Mic60 and Sam50

Autor: Ranjan Ramachandra, Mira Sastri, Manjula Darshi, Christopher Douglas, Saeyeon Ju, Susan S. Taylor, Jiwan John Kim, Kathryn Stein, Mason R. Mackey, Guy Perkins, Sebastien Phan, Mark H. Ellisman, Stephen R. Adams

Publikováno v: Journal of Cell Science.

Each mitochondrial compartment contains varying protein compositions that underlie a diversity of localized functions. Insights into the localization of mitochondrial intermembrane space-bridging (MIB) components will have an impact on our understand

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cc75e425e00176c7e13913f450778498
https://doi.org/10.1242/jcs.201400

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