Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Masayuki Yabuta"'
Autor:
Chie Nikaido, Ken Ishii, Masaki Imai, Keiji Oe, Toru Tanzawa, Yoshihiro Kawaoka, Masayuki Yabuta, Natsuki Tanaka, Takahiro Yano, Fumihiko Takeshita, Shizuko Ueno
Publikováno v:
Translational and Regulatory Sciences. 3:118-119
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::31a1949e2cc994f73d81e8a1c366edf9
https://doi.org/10.33611/trs.2021-020
https://doi.org/10.33611/trs.2021-020
Autor:
Masayuki Yabuta, Toyohiro Naito, Koji Otsuka, Kei Kubota, Takuya Kubo, Naoki Kobayashi, Motomu Ohara
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 140:98-104
This report describes a novel, comprehensive approach to identifying a fragment peak of monoclonal antibody-A (mAb-A), detected by sodium dodecyl sulfate-capillary gel electrophoresis (SDS-cGE). The fragment migrated close to the internal standard (1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c033dff299c2604709afb67e9c102208
https://doi.org/10.1016/j.jpba.2017.03.027
https://doi.org/10.1016/j.jpba.2017.03.027
Autor:
Motomu Ohara, Naoki Kobayashi, Kei Kubota, Masayuki Yabuta, Toyohiro Naito, Takuya Kubo, Koji Otsuka
Publikováno v:
CHROMATOGRAPHY. 37:117-124
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b80caadd8b207381422603e6512ba3d
https://doi.org/10.15583/jpchrom.2016.011
https://doi.org/10.15583/jpchrom.2016.011
Autor:
Takeshi Okumura, Koichi Nonaka, Masayuki Yabuta, Takeshi Omasa, Kenji Miyadai, Masuda Kenji, Watanabe Kazuhiko
Publikováno v:
Journal of Bioscience and Bioengineering. 120:340-346
To screen a high-producing recombinant Chinese hamster ovary (CHO) cell from transfected cells is generally laborious and time-consuming. We developed an efficient enrichment strategy for high-producing cell screening using flow cytometry (FCM). A st
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee85294a3e30dcf68689d86f66f8966d
https://doi.org/10.1016/j.jbiosc.2015.01.007
https://doi.org/10.1016/j.jbiosc.2015.01.007
Publikováno v:
Analytical Chemistry. 86:7536-7543
Although oxidation of methionine and tryptophan are known as popular chemical modifications that occur in monoclonal antibody (mAb) molecules, oxidation of other amino acids in mAbs has not been reported to date. In this study, oxidation of the histi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d28094c58340fc8482182efb8e207dcc
https://doi.org/10.1021/ac501300m
https://doi.org/10.1021/ac501300m
Autor:
Kimihisa Ichikawa, Rie Ito, Masayuki Yabuta, Minako Ono, Yasunori Chiba, Satoshi Baba, Takeshi Suzuki, Koichi Nonaka
Publikováno v:
Journal of bioscience and bioengineering. 124(2)
A production system for a therapeutic monoclonal antibody was developed using the methylotrophic yeast Ogataea minuta IFO10746. The genetically engineered O. minuta secreted a detectable amount of anti-TRAIL receptor antibody into the culture superna
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f90a0a8a6b6989a69af4cd7739b2398
https://pubmed.ncbi.nlm.nih.gov/28356218
https://pubmed.ncbi.nlm.nih.gov/28356218
Publikováno v:
Biotechnology journal. 12(1)
Protein A chromatography (PAC) is commonly used as an efficient capture step in monoclonal antibody (mAb) separation processes. Usually dynamic binding capacity is used for choosing the right PAC. However, if aggregates can be efficiently removed dur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::073ee717f45b7de57d4b965628df72df
https://pubmed.ncbi.nlm.nih.gov/27660109
https://pubmed.ncbi.nlm.nih.gov/27660109
Publikováno v:
Biosensors and Bioelectronics. 22:2308-2314
In our study, a method based on Enzymatic nanolithography was successfully performed in a buffered solution using Staphylococcal serine V8 protease and AFM. To estimate the lithographing activity of the protease immobilized on the AFM tip to peptides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76b37a36ef1095b251b21ce48a734ec1
https://doi.org/10.1016/j.bios.2006.12.026
https://doi.org/10.1016/j.bios.2006.12.026
Autor:
Tomohiro Makino, Katsuhiko Yamamoto, Yuji Suzuki, Masaru Matsumoto, Yasuo Kitajima, Yoshiharu Minamitake, Masashi Kuramoto, Masayuki Yabuta, Kenji Kangawa
Publikováno v:
Biopolymers. 79:238-247
The creation of peptide using a combination of recombinant expression and chemical synthesis can be a powerful tool for the production of a wide variety of polypeptides modified by phosphorylation, glycosylation, etc. We have developed a new method f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe0f8a8682a45ff2e5dd144a1c993519
https://doi.org/10.1002/bip.20342
https://doi.org/10.1002/bip.20342
Publikováno v:
Applied and Environmental Microbiology. 70:76-86
Escherichia coli outer-membrane endoprotease OmpT has suitable properties for processing fusion proteins to produce peptides and proteins. However, utilization of this protease for such production has been restricted due to its generally low cleavage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb586a17d598944d70dab5b7f4879352
https://doi.org/10.1128/aem.70.1.76-86.2004
https://doi.org/10.1128/aem.70.1.76-86.2004