Zobrazeno 1 - 10
of 85
pro vyhledávání: '"Masayasu Yamada"'
Publikováno v:
Reproductive Medicine and Biology, Vol 18, Iss 4, Pp 405-410 (2019)
Abstract Purpose Dimethyl α‐ketoglutarate (dm‐α‐KG) promotes in vitro development to blastocysts of C57BL/6J X C3He F1 mouse zygotes cultured in medium lacking pyruvate. Here, we examined the effects of pyruvate and dm‐α‐KG on in vitro d
Externí odkaz:
https://doaj.org/article/edbfc3dba91a4e4da957e96e5eb702a5
Autor:
Toshimichi ISHII, Koga KAWANO, Nobumasa TANAKA, Kensuke TOMITA, Naohiko SAITO, Masayasu YAMADA
Publikováno v:
The Journal of Reproduction and Development, Vol 65, Iss 3, Pp 275-280 (2019)
In this study, we evaluated the effects of holding in vitro-produced bovine blastocysts under mild hypothermia (33°C or 35°C), by examining viability and hatching rates of day 7 blastocysts (day 0: in vitro fertilization) cultured for 6 days and tr
Externí odkaz:
https://doaj.org/article/6b66027d55c54648880e6b0b08e5c460
Autor:
Kei Miyamoto, Yosuke Tajima, Koki Yoshida, Mami Oikawa, Rika Azuma, George E. Allen, Tomomi Tsujikawa, Tomomasa Tsukaguchi, Charles R. Bradshaw, Jerome Jullien, Kazuo Yamagata, Kazuya Matsumoto, Masayuki Anzai, Hiroshi Imai, John B. Gurdon, Masayasu Yamada
Publikováno v:
Biology Open, Vol 6, Iss 4, Pp 415-424 (2017)
Animal cloning has been achieved in many species by transplanting differentiated cell nuclei to unfertilized oocytes. However, the low efficiencies of cloning have remained an unresolved issue. Here we find that the combination of two small molecules
Externí odkaz:
https://doaj.org/article/8d8119656a7145dea3436bab1e00a2f6
Autor:
Takamasa Kawaguchi, Dooseon Cho, Masafumi Hayashi, Tomoyuki Tsukiyama, Koji Kimura, Shuichi Matsuyama, Naojiro Minami, Masayasu Yamada, Hiroshi Imai
Publikováno v:
PLoS ONE, Vol 11, Iss 12, p e0167550 (2016)
Trophectoderm lineage specification is one of the earliest differentiation events in mammalian development. The trophoblast lineage, which is derived from the trophectoderm, mediates implantation and placental formation. However, the processes involv
Externí odkaz:
https://doaj.org/article/a5c006c998e549faa27fbfc373d61352
Autor:
Takamasa Kawaguchi, Tomoyuki Tsukiyama, Koji Kimura, Shuichi Matsuyama, Naojiro Minami, Masayasu Yamada, Hiroshi Imai
Publikováno v:
PLoS ONE, Vol 10, Iss 8, p e0135403 (2015)
Generation of pluripotent stem cells (PSCs) in large domestic animals has achieved only limited success; most of the PSCs obtained to date have been classified as primed PSCs, which possess very little capacity to produce chimeric offspring. By contr
Externí odkaz:
https://doaj.org/article/4e77eff337f540e2aad59f5e17d98134
Publikováno v:
Reproductive Medicine and Biology
Reproductive Medicine and Biology, Vol 18, Iss 4, Pp 405-410 (2019)
Reproductive Medicine and Biology, Vol 18, Iss 4, Pp 405-410 (2019)
Purpose Dimethyl α‐ketoglutarate (dm‐α‐KG) promotes in vitro development to blastocysts of C57BL/6J X C3He F1 mouse zygotes cultured in medium lacking pyruvate. Here, we examined the effects of pyruvate and dm‐α‐KG on in vitro developmen
Publikováno v:
Molecular Reproduction and Development. 85(3):236-249
Undifferentiated spermatogonia eventually differentiate in the testis to produce haploid sperm. Within this cell population, there is a small number of spermatogonial stem cells (SSCs). SSCs are rare cells in the testis, and their cellular characteri
Publikováno v:
Journal of Visualized Experiments : JoVE
Somatic cell nuclear transfer (SCNT) provides a unique opportunity to directly produce a cloned animal from a donor cell, and it requires the use of skillful techniques. Additionally, the efficiencies of cloning have remained low since the successful
Effect of protopanaxatriol saponin on spermatogenic stem cell survival in busulfan-treated male mice
Publikováno v:
Reproductive medicine and biology. 6(2)
Background and aims: Panax ginseng C.A. Meyer is a medicinal herb widely used in Asian countries. Many of its pharmacological actions are attributed to ginsenosides (saponin). However, the pharmacological effects or functions of ginsenosides on mamma
Publikováno v:
The Journal of Reproduction and Development
In mouse somatic cell nuclear transfer (SCNT), polyvinylpyrrolidone (PVP) is typically included in the nuclear donor injection medium. However, the cytotoxicity of PVP, which is injected into the cytoplasm of oocytes, has recently become a cause of c