Not All Photoactive Yellow Proteins Are Built Alike: Surprises and Insights into Chromophore Photoisomerization, Protonation, and Thermal Reisomerization of the Photoactive Yellow Protein Isolated from

Autor: Sandip Kaledhonkar, Masato Kumauchi, Wouter D. Hoff, Miwa Hara, Aihua Xie, L. Tyler Mix, Delmar S. Larsen, Jack Fuzell

Publikováno v: Journal of the American Chemical Society. 143(46)

We demonstrate that the Halorhodospira halophila (Hhal) photoactive yellow protein (PYP) is not representative of the greater PYP family. The photodynamics of the PYP isolated from Salinibacter ruber (Srub) is characterized with a comprehensive range

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff559013c57c7cbd376d42f2524b6e55
https://pubmed.ncbi.nlm.nih.gov/34780163

Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration

Autor: Shojiro Haraguchi, Wouter D. Hoff, Masashi Unno, Tomotsumi Fujisawa, Takeshi Hanamoto, Noritaka Kasai, Takahito Shingae, Masato Kumauchi

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 115(35)

Photoactive yellow protein (PYP), from the phototrophic bacterium Halorhodospira halophila, is a small water-soluble photoreceptor protein and contains p-coumaric acid (pCA) as a chromophore. PYP has been an attractive model for studying the physical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc60959071dc6cca6db909ef5dddd082
https://pubmed.ncbi.nlm.nih.gov/30104345

Photo-isomerization upshifts the pKa of the Photoactive Yellow Protein chromophore to contribute to photocycle propagation

Autor: Wouter D. Hoff, Adèle D. Laurent, Elizabeth C. Carroll, Mikas Vengris, Anna I. Krylov, Masato Kumauchi, Delmar S. Larsen, Sadia Naseem

Publikováno v: Journal of Photochemistry and Photobiology A: Chemistry
Journal of Photochemistry and Photobiology A: Chemistry, Elsevier, 2013, 270, pp.43-52. ⟨10.1016/j.jphotochem.2013.06.019⟩

International audience; The influence of chromophore structure on the protonation constant of the Photoactive Yellow Protein chromophore is explored with isolated para-coumaric acid (pCA) and thiomethyl-para-coumaric acid model chromophores in soluti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32a520ca2dc9076672f335ce74f1b847
https://doi.org/10.1016/j.jphotochem.2013.06.019

Photoactive Yellow Protein Terahertz Response: Hydration, Heating and Intermediate States

Autor: Yunfen He, Deepu George, J. R. Knab, Wouter D. Hoff, Andrea Markelz, Robert R. Birge, Masato Kumauchi

Publikováno v: IEEE Transactions on Terahertz Science and Technology. 3:288-294

Photoactive yellow protein (PYP) is a model system for studies on functional protein dynamics and the role of protein flexibility during function. Functional conformational change is initiated by anharmonic collective vibrational modes that absorb in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dd40b9aded23fd06a06da2d43e9f06c8
https://doi.org/10.1109/tthz.2013.2256233

Exploring the Active Site Structure of a Photoreceptor Protein by Raman Optical Activity

Autor: Takashi Kikukawa, Masato Kumauchi, Masashi Unno, Naoki Kamo

Publikováno v: The Journal of Physical Chemistry B. 117:1321-1325

We have developed a near-infrared excited Raman optical activity (ROA) spectrometer and report the first measurement of near-infrared ROA spectra of a light-driven proton pump, bacteriorhodopsin. Our results demonstrate that a near-infrared excitatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb42a3e031b95a5f95a69358c1bf7b14
https://doi.org/10.1021/jp4001187