Zobrazeno 1 - 10 of 57 pro vyhledávání: '"Masataka, Nakagawa"'
1
Akademický článek
Polysaccharide as a Separation Medium for Gel Electrophoresis
Autor: Tsutomu Arakawa, Masataka Nakagawa, Chiaki Sakuma, Yui Tomioka, Yasunori Kurosawa, Teruo Akuta
Publikováno v: Polysaccharides, Vol 5, Iss 3, Pp 380-398 (2024)
Gel electrophoresis and size exclusion chromatography (SEC) are vital techniques in biochemical research, employing gel matrix structures made of polysaccharides or synthetic polymers like polyacrylamide for the analysis and separation of macromolecu
Externí odkaz: https://doaj.org/article/d7c938d31bd649848d40d0aecd02fd23
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2
Akademický článek
A case of pancreatic adenosquamous cell carcinoma with a pseudocyst following curative surgery
Autor: Nao Kitasaki, Tomoyuki Abe, Masashi Inoue, Marino Teshima, Masataka Nakagawa, Masatoshi Kochi, Ryuichi Hotta, Kazuhiro Toyota
Publikováno v: Surgical Case Reports, Vol 10, Iss 1, Pp 1-6 (2024)
Abstract Background Pancreatic adenosquamous cell carcinoma (PASC) is a relatively rare histological type of pancreatic malignancy, and preoperative diagnosis is difficult because of its rarity. PASC accounts for 1–4% of all pancreatic cancers, and
Externí odkaz: https://doaj.org/article/eedbc0a391ad45ad962b6c97308b2bc4
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3
Akademický článek
Western blotting of native proteins from agarose gels
Autor: Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Publikováno v: BioTechniques, Vol 72, Iss 5, Pp 207-218 (2022)
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provid
Externí odkaz: https://doaj.org/article/617c78fb8b3e44e2b265eac57674e82c
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4
Akademický článek
Non-Affinity Purification of Antibodies
Autor: Tsutomu Arakawa, Yui Tomioka, Masataka Nakagawa, Chiaki Sakuma, Yasunori Kurosawa, Daisuke Ejima, Kouhei Tsumoto, Teruo Akuta
Publikováno v: Antibodies, Vol 12, Iss 1, p 15 (2023)
Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are
Externí odkaz: https://doaj.org/article/cac5b1d7ce30488dba3045111abd0851
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5
Discontinuation of admission screening for coronavirus disease 2019 (COVID-19) and the impact on in-hospital clusters of COVID-19: Experience at a tertiary-care center
Autor: Hitoshi Honda, Masataka Nakagawa, Rie Nisida, Chiyo Shintani, Manami Hamagishi, Yuki Uehara, Mitsunaga Iwata, Yohei Doi
Publikováno v: Infection Control & Hospital Epidemiology. :1-4
We evaluated the impact of discontinuing universal preadmission screening for severe acute respiratory coronavirus virus 2 (SARS-CoV-2) on the occurrence of nosocomial clusters of coronavirus disease 2019 (COVID-19) during the SARS-CoV-2 o (omicron)
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a3558d6bb2effb54595b235193bad548
https://doi.org/10.1017/ice.2023.66
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6
Akademický článek
A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
Autor: Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa
Publikováno v: Antibodies, Vol 11, Iss 2, p 36 (2022)
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the
Externí odkaz: https://doaj.org/article/906228a9cdd34f64873d8605805429af
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10
Analysis of proteins by agarose native gel electrophoresis in the presence of solvent additives
Autor: Yui, Tomioka, Tsutomu, Arakawa, Teruo, Akuta, Masataka, Nakagawa, Matsujiro, Ishibashi
Publikováno v: International Journal of Biological Macromolecules. 198:26-36
Solvent additives, including NaCl, arginine hydrochloride (ArgHCl), glycine and sucrose, are used to enhance protein stability or reduce protein aggregation. Here, we studied the effects of these additives on proteins using agarose native gel electro
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b586972e79880738d2705067ba9943b9
https://doi.org/10.1016/j.ijbiomac.2021.12.084
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