Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Masaomi Arahira"'
Publikováno v:
European Journal of Biochemistry. 267:2649-2657
A soybean protein was purified from mature dry seeds. Amino-acid sequencing of the nine internal peptides derived from this N-terminally blocked protein showed that it has a significant similarity to the soluble epoxide hydrolases known to date. A de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::501f45759c4a98859f9ea4841504c5a0
https://doi.org/10.1046/j.1432-1327.2000.01276.x
https://doi.org/10.1046/j.1432-1327.2000.01276.x
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 63:726-730
Authentic soybean β-amylase preparation, purified to homogeneity as judged by SDS-PAGE by using an affinity purification step, was composed of four pI-differing isoforms. By chromatofocusing, these isoforms were separated into three fractions, desig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e78ba42b2f187a9452fb03ae463028e1
https://doi.org/10.1271/bbb.63.726
https://doi.org/10.1271/bbb.63.726
Autor:
Chikafusa Fukazawa, Van Hai Nong, Masaomi Arahira, Deyu Zhang, Akira Watanabe, Nana Asare Yeboah, Kazunari Kadokura
Publikováno v:
Plant Molecular Biology. 36:407-415
A soybean chitinase which has an apparent molecular mass of 28 kDa by SDS-PAGE, and has chitinase specific activity of 133 units per mg protein at pH 5.2 and an apparent pI of 5.7, was purified from mature dry seeds. Based upon the selected part (the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::388681a1f9c5d5a88c3a17e06e5eacf5
https://doi.org/10.1023/a:1005921104288
https://doi.org/10.1023/a:1005921104288
Publikováno v:
Plant Molecular Biology. 21:973-984
A 2.2 kb fragment containing the 5'-flanking region of the soybean glycinin A2B1a gene and its successive deletions with a shorter 5'-flanking sequence were fused, in frame, to the beta-glucuronidase (GUS) reporter gene. The resultant fusions were in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::855b18dcc27d9974d8546fd99359bedc
https://doi.org/10.1007/bf00023596
https://doi.org/10.1007/bf00023596
Publikováno v:
Journal of the Japanese Society of Starch Science. 38:197-200
Cyclodextrins (CDs, tt cyclomaltooligosaccharides) with maltosyl and panosyl branches were produced from maltose or panose and CDs by the reverse action of pullulanase. Purification on columns of octadesylated silica gave G2-α-CD, G2-β-CD, Pan-α-C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9cb9aaa0b79fc322824b9856fbb84015
https://doi.org/10.5458/jag1972.38.197
https://doi.org/10.5458/jag1972.38.197
Autor:
Van Hai Nong, Van Chi Phan, Chan-Shick Kim, Masaomi Arahira, Kyoko Udaka, Deyu Zhang, Chikafusa Fukazawa
Publikováno v:
Journal of biochemistry. 132(2)
Molecular characterization of plant group II chaperonin (CCT, c-cpn, or TriC) still remains elusive. By PCR-based cloning techniques using soybeans, we have made a successful attempt to clone a delta-subunit homologue of CCT (CCTdelta). This subunit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53d6a9d7c6ae30dc13c9f1f8ee8aac8e
https://pubmed.ncbi.nlm.nih.gov/12153728
https://pubmed.ncbi.nlm.nih.gov/12153728
Autor:
Akira Watanabe, Nana Asare Yeboah, Kyoko Udaka, Deyu Zhang, Chikafusa Fukazawa, Van Hai Nong, Masaomi Arahira
Publikováno v:
Bioscience, biotechnology, and biochemistry. 63(2)
A soybean seed-specific PR-8 chitinase, named Chib2, has a markedly extended C-terminal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date. To further characterize the molecular structure and the expression pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f75dc06877dbfefebe43618da4ae4d5
https://pubmed.ncbi.nlm.nih.gov/10192905
https://pubmed.ncbi.nlm.nih.gov/10192905
Molecular cloning and expression patterns of Cu/Zn-superoxide dismutases in developing soybean seeds
Autor:
Chikafusa Fukazawa, Kazunari Kadokura, Van Hai Nong, Keitarou Kimura, Masaomi Arahira, Kyoko Udaka
Publikováno v:
Bioscience, biotechnology, and biochemistry. 62(5)
Assuming that the amount of superoxide radicals generated in vivo correlates with the production of ergastic substances such as storage proteins, the coordinated response of detoxication enzymes such as superoxide dismutases is largely exploited to u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d2c7d42d48700aa7521b573f584d780
https://pubmed.ncbi.nlm.nih.gov/9648237
https://pubmed.ncbi.nlm.nih.gov/9648237
Publikováno v:
Protein expression and purification. 7(3)
A protein fraction was isolated from defatted soybean flour by extraction at acid pH, 40% ammonium sulfate precipitation, hydrophobic interaction chromatography, and gel filtration chromatography. SDS-PAGE, under reducing conditions, confirmed it as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b6a7f421b8a43273d0498c2356de465
https://pubmed.ncbi.nlm.nih.gov/8860657
https://pubmed.ncbi.nlm.nih.gov/8860657
Autor:
Chikafusa Fukazawa, Masaomi Arahira
Publikováno v:
Plant molecular biology. 25(4)
By reducing the amount of ginkgo water-soluble polysaccharides, which occupy about 35% of the wet seed mass and interfere with the extraction of RNA, cDNA-quality mRNA was obtained from developing seeds of Ginkgo biloba. Based on the NH2-terminal 17-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6d4da77c6088d691b030f97b5437a71
https://pubmed.ncbi.nlm.nih.gov/8061314
https://pubmed.ncbi.nlm.nih.gov/8061314