Zobrazeno 1 - 10
of 183
pro vyhledávání: '"Masao Tokunaga"'
Autor:
Takako Niikura, Tsutomu Arakawa, Richard W. Baker, Yoshiko Kita, J.M. Reimer, Andres E. Leschziner, Masao Tokunaga
Publikováno v:
The Protein Journal. 40:867-875
Difference circular dichroism (CD) spectroscopy was used here to characterize changes in structure of flexible peptides upon altering their environments. Environmental changes were introduced by binding to a large target structure, temperature shift
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5708edd6a49d558e0d92cec8f66fc27d
https://doi.org/10.1007/s10930-021-10024-7
https://doi.org/10.1007/s10930-021-10024-7
Autor:
Shunsuke Yamasaki, Tsutomu Arakawa, Hiroko Tokunaga, Ryoichi Tanaka, Matsujiro Ishibashi, Masao Tokunaga
Publikováno v:
International Journal of Biological Macromolecules. 164:77-86
Moderately halophilic bacterium, Chromohalobacter salexigens DSM3043, has a gene Csal_2537 encoding thermolysin-like M4 proteinase. This gene was cloned to pET expression vectors, resulting in high expression of recombinant proteinase, named as salil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::027c2a1b2becbcc669c192c570221b5d
https://doi.org/10.1016/j.ijbiomac.2020.07.050
https://doi.org/10.1016/j.ijbiomac.2020.07.050
Autor:
Rumi Shimizu, Chie Shibazaki, Hiroko Tokunaga, Matsujiro Ishibashi, Motoyasu Adachi, Shigeki Arai, Masao Tokunaga
Publikováno v:
Acta Crystallographica Section D Structural Biology. 76:73-84
Thioredoxin (TRX) is an important antioxidant against oxidative stress. TRX from the extremely halophilic archaeon Halobacterium salinarum NRC-1 (HsTRX-A), which has the highest acidic residue content [(Asp + Glu)/(Arg + Lys + His) = 9.0] among known
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28ee01dbbe280977697c2159394114f9
https://doi.org/10.1107/s2059798319015894
https://doi.org/10.1107/s2059798319015894
Autor:
Tsutomu, Arakawa, Masao, Tokunaga, Yoshiko, Kita, Takako, Niikura, Richard W, Baker, Janice M, Reimer, Andres E, Leschziner
Publikováno v:
The protein journal. 40(6)
Difference circular dichroism (CD) spectroscopy was used here to characterize changes in structure of flexible peptides upon altering their environments. Environmental changes were introduced by binding to a large target structure, temperature shift
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a3858707de8fecdf714cfabf1f767fd2
https://pubmed.ncbi.nlm.nih.gov/34709521
https://pubmed.ncbi.nlm.nih.gov/34709521
Autor:
Ryoichi Tanaka, Tsutomu Arakawa, Masao Tokunaga, Shunsuke Yamasaki, Hiroko Tokunaga, Matsujiro Ishibashi
Publikováno v:
The protein journal. 40(2)
Halophilic salilysin is first synthesized as a pro-form, which has been shown autolysis activity to process pro-region (55 amino acids long) three times to form intermediate 1 (I1), intermediate 2 (I2) and final mature (M) salilysin. The autolysis of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::557165876cd39c755d7733336f6c36f7
https://pubmed.ncbi.nlm.nih.gov/33502674
https://pubmed.ncbi.nlm.nih.gov/33502674
Autor:
Tsutomu Arakawa, Akira Miyauchi, Hiroshi Hanagata, Hiroko Tokunaga, Makoto Mizukami, Yuji Ito, Matsujiro Ishibashi, Hiromasa Onishi, Masao Tokunaga
Publikováno v:
Protein Expression and Purification. 150:109-118
The Brevibacillus expression system has been successfully employed for the efficient productions of a variety of recombinant proteins, including enzymes, cytokines, antigens and antibody fragments. Here, we succeeded in secretory expression of Trastu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e31787d905963e628037d779678b9817
https://doi.org/10.1016/j.pep.2018.05.013
https://doi.org/10.1016/j.pep.2018.05.013
Autor:
Fina Amreta Laksmi, Norie Amaba, Matsujiro Ishibashi, Masao Tokunaga, Shigeki Arai, Kenta Komori, Ryo Kawanabe
Publikováno v:
Protein Expression and Purification. 145:39-44
Luciferase from Renilla reniformis (RLuc) is a good research tool as a reporter protein and bioimaging probes, yielding blue light using the substrate coelenterazine. However, the applications are limited since RLuc is unstable under various conditio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b35204161b229b5b0d19ea4b61202e5c
https://doi.org/10.1016/j.pep.2017.12.010
https://doi.org/10.1016/j.pep.2017.12.010
Autor:
Fina Amreta Laksmi, Masao Tokunaga, Yoshitaka Nakamura, Shigeki Arai, Matsujiro Ishibashi, Hirohito Tsurumaru, Budi Saksono, Tsutomu Arakawa
Publikováno v:
Protein expression and purification. 175
A low-calorie sugar-substituting sweetener, d-tagatose, can be produced by l-arabinose isomerase (l-AI) from the substrate d-galactose. However, this process suffers from a Maillard reaction when performed at alkaline pH and high temperature. For ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67e4e124ae949f35de8409dc97796a5e
https://pubmed.ncbi.nlm.nih.gov/32681957
https://pubmed.ncbi.nlm.nih.gov/32681957
Autor:
Yoshitaka Nakamura, Hiroshi Hanagata, Hirohito Tsurumaru, Shigeki Arai, Matsujiro Ishibashi, Fina Amreta Laksmi, Hikari Imamura, Masao Tokunaga
Publikováno v:
The protein journal. 39(1)
Halophilic enzymes contain a large number of acidic amino acids and marginal large hydrophobic amino acids, which make them highly soluble even under strongly hydrophobic conditions. This characteristic of halophilic enzymes provides potential for th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8018349ef25da5ef6867773832851f04
https://pubmed.ncbi.nlm.nih.gov/31734848
https://pubmed.ncbi.nlm.nih.gov/31734848
Publikováno v:
Protein expression and purification. 156
Insoluble expression of intrinsically soluble proteins with native activity is potentially a promising alternative to soluble expression of folded protein or insoluble expression of unfolded protein requiring refolding. Here, we attempted to express
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::516362b5cc27ec5b1884bd506584da27
https://pubmed.ncbi.nlm.nih.gov/30615940
https://pubmed.ncbi.nlm.nih.gov/30615940