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pro vyhledávání: '"Masanaru Ozaki"'
Publikováno v:
Biocatalysis and Biotransformation. 34:249-252
An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that ha
Publikováno v:
Biocatalysis and Biotransformation. 33:188-190
A GGG(A)X-type esterase (Est0071) from an archaeon catalyzes asymmetric hydrolysis of prochiral bulky malonic diesters in good enantioselectivity. The selectivity of Est0071 was for the opposite enantiomer to that previously shown for pig liver ester