Zobrazeno 1 - 10
of 242
pro vyhledávání: '"Masahiko Sisido"'
Autor:
Takashi Ohtsuki, Shigeto Kanzaki, Sae Nishimura, Yoshio Kunihiro, Masahiko Sisido, Kazunori Watanabe
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
Spatiotemporal regulation of protein synthesis would advance studies into the consequences of localised protein translation in cells and tissues. Here, Ohtsuki et al.improve on an earlier caged-tRNA design to provide caged aminoacyl-tRNAs that are ra
Externí odkaz:
https://doaj.org/article/8b762495f9ea4fd1bd661adc3e2c50e9
Autor:
Yoshio Kunihiro, Masahiko Sisido, Sae Nishimura, Kazunori Watanabe, Takashi Ohtsuki, Shigeto Kanzaki
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
Nature Communications
Nature Communications
The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethyla
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1130dcc1160b520e5e72ba926ae0dfc
https://doaj.org/article/8b762495f9ea4fd1bd661adc3e2c50e9
https://doaj.org/article/8b762495f9ea4fd1bd661adc3e2c50e9
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 24:5369-5372
Translation systems with four-base codons provide a powerful strategy for protein engineering and protein studies because they enable site-specific incorporation of non-natural amino acids into proteins. In this study, a caged aminoacyl-tRNA with a f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d01a34c0a6879da92377af77a8f4736
https://doi.org/10.1016/j.bmcl.2014.10.053
https://doi.org/10.1016/j.bmcl.2014.10.053
Publikováno v:
Biochemical and Biophysical Research Communications. 414:625-630
Arginine analogs were incorporated site-specifically into proteins using an in vitro translation system. In this system, mRNAs containing a CGGG codon were translated by an aminoacyl-tRNA(CCCG), which was charged with arginine analogs using yeast arg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9672851ac62dd497f5aa27af5ee7f566
https://doi.org/10.1016/j.bbrc.2011.09.137
https://doi.org/10.1016/j.bbrc.2011.09.137
Publikováno v:
Tetrahedron. 66:9659-9666
We synthesized 16 pyrrolidine-based oxy-peptide nucleic acid (POPNA) monomers carrying four different nucleobases onto four different stereoisomers of pyrrolidine rings. Using these monomers, we prepared POPNA oligomers, which formed sequence-specifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d4cf6afc0f86019737a010ad70c583f
https://doi.org/10.1016/j.tet.2010.10.056
https://doi.org/10.1016/j.tet.2010.10.056
Publikováno v:
Journal of Biochemistry. 148:239-246
We present three methods relating to tRNA aminoacylation with non-natural amino acids using an Escherichia coli EF-Tu E215A/D216A mutant that can bind tightly to aa-tRNAs carrying either non-natural or natural amino acids: (i) a method for improving
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b7526ce150cc2c0d1843b3a46ab7b84
https://doi.org/10.1093/jb/mvq053
https://doi.org/10.1093/jb/mvq053
Publikováno v:
Journal of Bioscience and Bioengineering. 109:189-192
We engineered Lactobacillus paracasei to produce a dsRNA that would trigger RNAi-induced silencing of an essential gene in the nematode Caenorhabditis elegans. The dsRNA-expressing L. paracasei can be used in experiments conducted on culture plates a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::925804b692dcf96c404b306f941941d8
https://doi.org/10.1016/j.jbiosc.2009.08.002
https://doi.org/10.1016/j.jbiosc.2009.08.002
Publikováno v:
Journal of Controlled Release. 137:241-245
In this study we describe the spatial regulation of RNA interference (RNAi) using an RNA-carrier protein labeled with a fluorescent dye and a light source to trigger the RNAi. We demonstrate photo-dependent gene silencing using several dyes with diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64d5c701253a43b491cefc652e8c4a4d
https://doi.org/10.1016/j.jconrel.2009.04.015
https://doi.org/10.1016/j.jconrel.2009.04.015
Autor:
Masahiko Sisido, Tamaki Endoh, Rino Matsuzaki, Mizuki Kitamatsu, Takanori Kubo, Takashi Ohtsuki
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 19:3410-3413
A peptide nucleic acid (PNA)-cell-penetrating peptide (CPP) conjugate (carrier PNA) was used as 'bridge-builder' to connect a CPP with an shRNA. The carrier PNA successfully formed a hybrid with an shRNA bearing complementary dangling bases and the s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76d8620647f4e727f579bb73ef35d325
https://doi.org/10.1016/j.bmcl.2009.05.031
https://doi.org/10.1016/j.bmcl.2009.05.031
Autor:
Takashi Ohtsuki, Takeshi Fujimoto, Masahiko Sisido, Chisato Kumano, Mizuki Kitamatsu, Maya Kamimukai
Publikováno v:
The Journal of Biochemistry. 144:415-418
In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from huma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18c53cc4acb904650d6d88d56265f05e
https://doi.org/10.1093/jb/mvn107
https://doi.org/10.1093/jb/mvn107