Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Masahide Ishikawa"'
Publikováno v:
In Journal of Environmental Sciences December 2013 25 Supplement 1:S169-S171
Publikováno v:
Journal of Materials Science and Chemical Engineering. :13-18
We studied the relationship between insertion of the last sense codon (i.e., the codon preceding the stop codon) and the efficiency of gene expression. We inserted 64 kinds of last sense codon at the 5’ end of the stop codon of the green fluorescen
Autor:
Satoshi Suzuki, Takeshi Ohoka, Shun Sakuma, Tomohiro Nakahata, Norikazu Matsumura, Masahide Ishikawa
Publikováno v:
Journal of environmental sciences (China). 21
Enzymes fixed on the electrode of biosensor are gradually inactivated and the electrode is discarded after using several times. In order to prepare the stable biosensor, we try to use a stable enzyme from extreme thermophilic bacteria, Thermus thermo
Autor:
Mitsuo Sekine, Akira Mochizuki, Seiichiro Higashiya, Hiroyuki Tsuruoka, Takeshi Wada, Masahide Ishikawa, Shun-ichi Kawahara
Publikováno v:
Tetrahedron Letters. 42:9215-9219
2-Azidodeoxyadenosine ( 7 ) was conveniently synthesized from deoxyguanosine ( 2 ) by use of a combined reagent of TMSN 3 –BuONO. The structure of the tautomer of the azido derivative was determined by 1 H NMR. Reaction of 7 with i Pr 2 NP(OEt) 2 g
Autor:
Susumu Nakada, Motoki Tado, Toshifumi Hatta, Takayuki Abe, Hiroshi Takaku, Tomoyuki Yokota, Masahide Ishikawa
Publikováno v:
Antiviral Chemistry and Chemotherapy. 12:353-358
We have shown previously that the 5′-capped short phosphodiester RNA fragments, Cap decoy, (Gm 12 nt) are potent inhibitors of influenza virus RNA polymerase gene expression. Here we investigate the modified capped RNA derivative containing phospho
Autor:
Masahide Ishikawa, Takashi Ohtsuki, Shigeyuki Yokoyama, Tsuneo Mitsui, Ichiro Hirao, Michiko Kimoto
Publikováno v:
Proceedings of the National Academy of Sciences. 98:4922-4925
An unnatural base pair of 2-amino-6-( N , N -dimethylamino)purine (designated as x) and pyridin-2-one (designated as y) has been developed for specific transcription. The ribonucleoside triphosphates of y and a modified y, 5-methylpyridin-2-one, are
Autor:
Chitose Kaibara, Seiichiro Higashiya, Fuminori Suda, Masahide Ishikawa, Koichiro Fukuoka, Masasuke Yoshida, Tsujiaki Hata
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 6:39-42
2-Azidoadenosine ( 1 ) was synthesized in an overall yield of 49% from guanosine via the reaction of 9-(2′,3′,5′-tri- O -acetyl- β -D-ribofuranosyl)-2-amino-6-chloropurine ( 2 ) with isoamyl nitrite and trimethylsilyl azide under neutral and a
Autor:
Masahide Ishikawa
Publikováno v:
JOURNAL OF THE ILLUMINATING ENGINEERING INSTITUTE OF JAPAN. 87:182-184
Autor:
Masahide Ishikawa, Tsujiaki Hata, Hiroshi Takaku, Koichiro Fukuoka, Fuminori Suda, Ryo Suzuki
Publikováno v:
Nucleosides and Nucleotides. 13:1557-1567
Bifunctional phosphorylating reagents 1 and 2 were employed for the synthesis of the cap part, m7G7 pppG, from guanosine 5′-phosphates on a large scale without any protecting groups.
Publikováno v:
Chemistry Letters. 23:499-502
S,S′-Bis(4-chlorophenyl) phosphorodithioate was useful for the synthesis of α,γ-dinucleoside 5′-triphosphates, G5′pppG and A5′pppA starting from the corresponding unprotected nucleoside 5′-phosphates under neutral conditions. G5′pppG wa