Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325° to 333deg

Autor: M.-P. Coudart, Margarida Santana, Georges Rapoport, J. Schwelzer, I. Moszer, Luisa A. Marcelino, E. Presecan, Maryvonne Arnaud, Antoine Danchin, Marie-Françoise Hullo, E. Schneider, B. Lubochinsicy, W. Gonzales, A. Vertes, F. Kunst, M S Ionescu, Philippe Glaser

Publikováno v: Molecular microbiology. 10(2)

In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314 degrees) and sacXY (333 degrees) was assigned to the Institut Pasteur. In this paper we describe the cloning and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a386f04defeea3a3f50624d340e21c84
https://pubmed.ncbi.nlm.nih.gov/28776854

New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria

Autor: Maryvonne Arnaud, Michel Débarbouillé, Arnaud Chastanet

Publikováno v: Applied and Environmental Microbiology
Applied and Environmental Microbiology, American Society for Microbiology, 2004, 70 (11), pp.6887-6891. ⟨10.1128/AEM.70.11.6887-6891.2004⟩
Applied and Environmental Microbiology 11 (70), 6887-6891. (2004)

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-β- d- galactopyranoside) plates, a quick

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03106ce16bae2767b76af998234b2e82
https://hal.inrae.fr/hal-02681992/document

PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria

Autor: Isabelle Martin-Verstraete, Maryvonne Arnaud, Georges Rapoport, Jörg Stülke

Publikováno v: Molecular microbiology. 28(5)

Several operon-specific transcriptional regulators, including antiterminators and activators, contain a duplicated conserved domain, the PTS regulation domain (PRD). These duplicated domains modify the activity of the transcriptional regulators both

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5491ea75d460cc9f940721d6488b63f9
https://pubmed.ncbi.nlm.nih.gov/9663674

Regulation of the sacPA operon of Bacillus subtilis : identification of phosphotransferase system components involved in SacT activity

Autor: Michel Débarbouillé, Maryvonne Arnaud, Monique Zagorec, P Postma, A Klier, P Vary, Georges Rapoport

Publikováno v: Journal of Bacteriology
Journal of Bacteriology, American Society for Microbiology, 1992, 174 (10), pp.3161-3170
HAL

The sacT gene which controls the sacPA operon of Bacillus subtilis encodes a polypeptide homologous to the B. subtilis SacY and the Escherichia coli BglG antiterminators. Expression of the sacT gene is shown to be constitutive. The DNA sequence upstr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439b1aa03c82ce2aec272c8672192e34
https://hal.inrae.fr/hal-02714726

The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators

Autor: Maryvonne Arnaud, Agnès Fouet, Georges Rapoport, A Klier, Michel Débarbouillé

The expression of the Bacillus subtilis sacPA operon is induced by sucrose. A DNA fragment containing the upstream region of this operon was cloned. This fragment contains a promoter from which the operon is expressed. This upstream region also conta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdce505cea829d042537defccae539db
https://europepmc.org/articles/PMC213381/