TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway

Autor: Nila Roy Choudhury, Ivan Trus, Gregory Heikel, Magdalena Wolczyk, Jacek Szymanski, Agnieszka Bolembach, Rute Maria Dos Santos Pinto, Nikki Smith, Maryia Trubitsyna, Eleanor Gaunt, Paul Digard, Gracjan Michlewski

Publikováno v: Choudhury, N R, Trus, I, Heikel, G, Wolczyk, M, Szymanski, J, Bolembach, A, Dos santos pinto, R M, Smith, N, Trubitsyna, M, Gaunt, E, Digard, P & Michlewski, G 2022, ' TRIM25 inhibits influenza A virus infection, destabilizes viral mRNA, but is redundant for activating the RIG-I pathway ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkac512
Roy Choudhury, N, trus, I, Heikel, G, Wolczyk, M, Szymanski, J, Bolembach, A, Dos Santos Pinto, R M, Smith, N, Trubitsyna, M, Gaunt, E, Digard, P & Michlewski, G 2022, ' TRIM25 inhibits influenza A virus infection, destabilises viral mRNA, but is redundant for activating the RIG-I pathway ', Nucleic Acids Research, vol. 50, no. 12, pp. 7097-7114 . https://doi.org/10.1093/nar/gkac512

The E3 ubiquitin ligase TRIM25 is a key factor in the innate immune response to RNA viruses. TRIM25 has been shown to play a role in the retinoic-acid-inducible gene-1 (RIG-I) pathway, which triggers expression of type 1 interferons upon viral infect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0436a24b5e6b20022dd685abfc472163
https://doi.org/10.1093/nar/gkac512

PaperClip DNA Assembly: Reduce, Reuse, Recycle

Autor: Maryia, Trubitsyna, Annegret, Honsbein, Uma, Jayachandran, Alistair, Elfick, Christopher E, French

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2205

Creating DNA constructs is a basic and fundamental step in molecular and synthetic biology. While prices for gene synthesis are decreasing, it is still more economical in most cases to assemble constructs from a library of components (Parts). Many me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1da43d99b13f1f93a9fb63218b8fdad3
https://pubmed.ncbi.nlm.nih.gov/32809199

RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination

Autor: Alfredo Castello, Christos Spanos, Nila Roy Choudhury, Peter Kubik, Juri Rappsilber, Shaun Webb, Sander Granneman, Gracjan Michlewski, Maryia Trubitsyna, Gregory Heikel, Jakub Stanislaw Nowak

Publikováno v: BMC Biology, Vol 15, Iss 1, Pp 1-20 (2017)
BMC Biology
Choudhury, N R, Heikel, G, Trubitsyna, M, Kubik, P, Nowak, J S, Webb, S, Granneman, S, Spanos, C, Rappsilber, J, Castello, A & Michlewski, G 2017, ' RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination ', BMC Biology, vol. 15, 105, pp. 105-126 . https://doi.org/10.1186/s12915-017-0444-9

Background TRIM25 is a novel RNA-binding protein and a member of the Tripartite Motif (TRIM) family of E3 ubiquitin ligases, which plays a pivotal role in the innate immune response. However, there is scarce knowledge about its RNA-related roles in c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b45757dcd1f7a88d814b282bdb68fc2
https://doi.org/10.1101/200410

PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly

Autor: Maryia, Trubitsyna, Chao-Kuo, Liu, Alejandro, Salinas, Alistair, Elfick, Christopher E, French

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1472

Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0b89ad09f4255ce5761162a2e2939dd8
https://pubmed.ncbi.nlm.nih.gov/27671936

Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection

Autor: Maryia, Trubitsyna, Gracjan, Michlewski, David J, Finnegan, Alistair, Elfick, Susan J, Rosser, Julia M, Richardson, Christopher E, French

Publikováno v: Nucleic Acids Research

Delivery of DNA to cells and its subsequent integration into the host genome is a fundamental task in molecular biology, biotechnology and gene therapy. Here we describe an IP-free one-step method that enables stable genome integration into either pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d9100c6e59dc22e19fe22ffacc799dc0
https://pubmed.ncbi.nlm.nih.gov/28204586